Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes

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題名:	Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes
作者:	樋口亞紺;Chen, Da-Chung;Chen, Li-Yu;Ling, Qing-Dong;Wu, Meng-Hsueh;Wang, Ching-Tang;Suresh Kumar, S.;Chang, Yung;Munusamy, Murugan A.;Alarfajj, Abdullah A.;Wang, Han-Chow;Hsu, Shih-Tien;Higuchi, Akon
貢獻者:	工學院化學工程與材料工程學系
關鍵詞:	adipose tissue;Adipose Tissue - cytology;Adipose-derived stem cells;Advanced Basic Science;Aged;Aged, 80 and over;alizarin;Animals;Biomarkers - metabolism;Bioseparation;bone formation;Cell Count;Cell Differentiation - drug effects;Cell Membrane Permeability - drug effects;Cell Separation;Dentistry;Differentiation;enzyme activity;Filtration;Flow Cytometry;gene expression;Humans;Lactic Acid - pharmacology;medicine;Membrane purification;Membranes, Artificial;Mesenchymal Stromal Cells - cytology;Mesenchymal Stromal Cells - drug effects;Mesenchymal Stromal Cells - metabolism;microfiltration;Microscopy, Electron, Scanning;Middle Aged;mineralization;Osteoblast;Osteoblasts - cytology;Osteoblasts - drug effects;Osteoblasts - metabolism;Osteogenesis - drug effects;Osteogenesis - genetics;Poly(lactide-co-glycolic acid);Polyglycolic Acid - pharmacology;silk;Silk - pharmacology;Solutions;staining;stem cells;Stem Cells - cytology;Stem Cells - drug effects;Stem Cells - metabolism;Stromal Cells - drug effects;Stromal Cells - metabolism;Subcellular Fractions - drug effects;Subcellular Fractions - metabolism
日期:	2014-05-01
上傳時間:	2026-04-21 14:25:40 (UTC+8)
出版者:	Elsevier BV;Netherlands: Elsevier Ltd
摘要:	摘要： The purification of human adipose-derived stem cells (hADSCs) from human adipose tissue cells (stromal vascular fraction) was investigated using membrane filtration through poly(lactide-co-glycolic acid)/silk screen hybrid membranes. Membrane filtration methods are attractive in regenerative medicine because they reduce the time required to purify hADSCs (i.e., less than 30 min) compared with conventional culture methods, which require 5–12 days. hADSCs expressing the mesenchymal stem cell markers CD44, CD73, and CD90 were concentrated in the permeation solution from the hybrid membranes. Expression of the surface markers CD44, CD73, and CD99 on the cells in the permeation solution from the hybrid membranes, which were obtained using 18 mL of feed solution containing 50 × 104 cells, was statistically significantly higher than that of the primary adipose tissue cells, indicating that the hADSCs can be purified in the permeation solution by the membrane filtration method. Cells expressing the stem cell-associated marker CD34 could be successfully isolated in the permeation solution, whereas CD34+ cells could not be purified by the conventional culture method. The hADSCs in the permeation solution demonstrated a superior capacity for osteogenic differentiation based on their alkali phosphatase activity, their osterix gene expression, and the results of mineralization analysis by Alizarin Red S and von Kossa staining compared with the cells from the suspension of human adipose tissue. These results suggest that the hADSCs capable of osteogenic differentiation preferentially permeate through the hybrid membranes. 其他題名： Biomaterials 出版者： Netherlands: Elsevier Ltd 出版日期： 2014-05-01 出處： Biomaterials, 2014-05, Vol.35 (14), p.4278-4287 版權： 2014 Elsevier Ltd 版權： Elsevier Ltd 版權： Copyright © 2014 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0142-9612 識別號： ISSN: 1878-5905 識別號： EISSN: 1878-5905 識別號： DOI: 10.1016/j.biomaterials.2014.02.004 識別號： PMID: 24565521
顯示於類別:	[化學工程與材料工程學系 ] 期刊論文

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