Developing antifouling biointerfaces based on bioinspired zwitterionic dopamine through pH-modulated assembly

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Title:	Developing antifouling biointerfaces based on bioinspired zwitterionic dopamine through pH-modulated assembly
Authors:	黃俊仁;Huang, Chun-Jen;Wang, Lin-Chuan;Shyue, Jing-Jong;Chang, Ying-Chih
Contributors:	工學院化學工程與材料工程學系
Keywords:	Adsorption;Animals;bacteria;Bacterial Adhesion;Betaine - analogs & derivatives;Betaine - chemistry;biocompatible materials;bovine serum albumin;Cattle;Coated Materials, Biocompatible - chemistry;coatings;contact angle;dopamine;Dopamine - chemistry;electrochemistry;fluorescence;fouling;hydrogen bonding;Hydrogen-Ion Concentration;ligands;medical equipment;moieties;mussels;Pseudomonas aeruginosa - cytology;Pseudomonas aeruginosa - metabolism;quartz crystal microbalance;Serum Albumin, Bovine - chemistry;Staphylococcus epidermidis - cytology;Staphylococcus epidermidis - metabolism;Surface Properties;thrombosis;Titanium;titanium dioxide;X-ray photoelectron spectroscopy;zwitterions
Date:	2014-10-28
Issue Date:	2026-04-21 14:29:18 (UTC+8)
Publisher:	American Chemical Society;United States: American Chemical Society
Abstract:	摘要： The use of synthetic biomaterials as implantable devices typically is accompanied by considerable nonspecific adsorption of proteins, cells, and bacteria. These may eventually induce adverse pathogenic problems in clinical practice, such as thrombosis and biomaterial-associated infection. Thus, an effective surface coating for medical devices has been pursued to repel nonspecific adsorption from surfaces. In this study, we employ an adhesive dopamine molecule conjugated with zwitterionic sulfobetaine moiety (SB-DA), developed based on natural mussels, as a surface ligand for the modification of TiO2. The electrochemical study shows that the SB-DA exhibits fully reversible reduction–oxidation behavior at pH 3, but it is irreversible at pH 8. A contact angle goniometer and X-ray photoelectron spectroscopy were utilized to explore the surface hydration, chemical states, and bonding mechanism of SB-DA. The results indicate that the binding between hydroxyl groups of SB-DA and TiO2 converts from hydrogen bonds to bidentate binding upon the pH transition from pH 3 to 8. In order to examine the antifouling properties of SB-DA thin films, the modified substrates were brought into contact with bovine serum albumin and bacteria solutions. The fouling levels were monitored using a quartz crystal microbalance with dissipation sensor and fluorescence optical microscope. Tests showed that the sample prepared via the pH transition approach provides the best resistance to nonspecific adsorption due to the high coverage and stability of the SB-DA films. These findings support the mechanism of the pH-modulated assembly of SB-DA molecules, and for the first time we demonstrate the antifouling properties of the SB-DA to be comparable with traditional thiol-based zwitterionic self-assemblies. The success of modification with SB-DA opens an avenue for developing a biologically inspired surface chemistry and can have applications over a wide spectrum of bioapplications. The strategy of the pH transition can also be applied to other functional dopamine derivatives. 其他題名： Langmuir 出版者： United States: American Chemical Society 出版日期： 2014-10-28 出處： Langmuir, 2014-10, Vol.30 (42), p.12638-12646 資源來源： American Chemical Society Journals 識別號： ISSN: 0743-7463 識別號： ISSN: 1520-5827 識別號： EISSN: 1520-5827 識別號： DOI: 10.1021/la503191b 識別號： PMID: 25283175
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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