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| 題名: | Protein-silver nanoparticle interactions to colloidal stability in acidic environments |
| 作者: | 王孝方;Tai, Jui-Ting;Lai, Chao-Shun;Ho, Hsin-Chia;Yeh, Yu-Shan;Wang, Hsiao-Fang;Ho, Rong-Ming;Tsai, De-Hao |
| 貢獻者: | 工學院化學工程與材料工程學系 |
| 關鍵詞: | acidity;Animals;binding capacity;bovine serum albumin;Cattle;Colloids;Drug Stability;Hydrogen-Ion Concentration;Kinetics;Metal Nanoparticles - chemistry;Models, Molecular;nanoparticles;nanosilver;particle size;particle size distribution;Protein Binding;Protein Conformation;Serum Albumin, Bovine - chemistry;silver;Silver - chemistry;transmission electron microscopy |
| 日期: | 2014-11-04 |
| 上傳時間: | 2026-04-21 14:29:27 (UTC+8) |
| 出版者: | American Chemical Society;United States: American Chemical Society |
| 摘要: | 摘要： We report a kinetic study of Ag nanoparticles (AgNPs) under acidic environments (i.e., pH 2.3 to pH ≈7) and systematically investigate the impact of protein interactions [i.e., bovine serum albumin (BSA) as representative] to the colloidal stability of AgNPs. Electrospray-differential mobility analysis (ES-DMA) was used to characterize the particle size distributions and the number concentrations of AgNPs. Transmission electron microscopy was employed orthogonally to provide visualization of AgNPs. For unconjugated AgNPs, the extent of aggregation, or the average particle size, was shown to be increased significantly with an increase of acidity, where a partial coalescence was found between the primary particles of unconjugated AgNP clusters. Aggregation rate constant, k D, was also shown to be proportional to acidity, following a correlation of log(k D) = −1.627(pH)–9.3715. Using ES-DMA, we observe BSA had a strong binding affinity (equilibrium binding constant, ≈ 1.1 × 106 L/mol) to the surface of AgNPs, with an estimated maximum molecular surface density of ≈0.012 nm–2. BSA-functionalized AgNPs exhibited highly-improved colloidal stability compared to the unconjugated AgNPs under acidic environments, where both the acid-induced interfacial dissolution and the particle aggregation became negligible. Results confirm a complex mechanism of colloidal stability of AgNPs: the aggregation process was shown to be dominant, and the formation of BSA corona on AgNPs suppressed both particle aggregation and interfacial dissolution of AgNP samples under acidic environments.
其他題名： Langmuir
出版者： United States: American Chemical Society
出版日期： 2014-11-04
出處： Langmuir, 2014-11, Vol.30 (43), p.12755-12764
資源來源： American Chemical Society Journals
識別號： ISSN: 0743-7463
識別號： ISSN: 1520-5827
識別號： EISSN: 1520-5827
識別號： DOI: 10.1021/la5033465
識別號： PMID: 25294101 |
| 顯示於類別: | [化學工程與材料工程學系 ] 期刊論文
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