Catalysis of rice straw hydrolysis by the combination of immobilized cellulase from aspergillus niger on β -Cyclodextrin-Fenanoparticles and ionic liquid

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題名:	Catalysis of rice straw hydrolysis by the combination of immobilized cellulase from aspergillus niger on β -Cyclodextrin-Fenanoparticles and ionic liquid
作者:	黃柏榮;Chen, Shui-Tein;Hsieh, Jung-Feng;Chang, Ken-Lin;Huang, Po-Jung
貢獻者:	工學院化學工程與材料工程學系
關鍵詞:	Aspergillus niger - enzymology;beta-Cyclodextrins - chemistry;Cellulase - chemistry;Enzymes, Immobilized - chemistry;Ferrosoferric Oxide - chemistry;Fungal Proteins - chemistry;Ionic Liquids - chemistry;Nanoparticles - chemistry;Oryza - chemistry
日期:	2015-01-01
上傳時間:	2026-04-21 14:30:48 (UTC+8)
出版者:	Hindawi Publishing Corporation;Cairo, Egypt: Hindawi Publishing Corporation
摘要:	摘要： Cellulase from Aspergillus niger was immobilized onto β-cyclodextrin-conjugated magnetic particles by silanization and reductive amidation. The immobilized cellulase gained supermagnetism due to the magnetic nanoparticles. Ninety percent of cellulase was immobilized, but the activity of immobilized cellulase decreased by 10%. In this study, ionic liquid (1-butyl-3-methylimidazolium chloride) was introduced into the hydrolytic process because the original reaction was a solid-solid reaction. The activity of immobilized cellulase was improved from 54.87 to 59.11 U g immobilized cellulase−1 at an ionic liquid concentration of 200 mM. Using immobilized cellulase and ionic liquid in the hydrolysis of rice straw, the initial reaction rate was increased from 1.629 to 2.739 g h−1 L−1. One of the advantages of immobilized cellulase is high reusability—it was usable for a total of 16 times in this study. Compared with free cellulase, magnetized cellulase can be recycled by magnetic field and the activity of immobilized cellulase was shown to remain at 85% of free cellulase without denaturation under a high concentration of glucose (15 g L−1). Therefore, immobilized cellulase can hydrolyze rice straw continuously compared with free cellulase. The amount of harvested glucose can be up to twentyfold higher than that from the hydrolysis by free cellulase. 其他題名： Biomed Res Int 出版者： Cairo, Egypt: Hindawi Publishing Corporation 出版日期： 2015-01-01 出處： BioMed research international, 2015-01, Vol.2015 (2015), p.1-9 資源來源： Publicly Available Content Database 版權： Copyright © 2015 Po-Jung Huang et al. 版權： Copyright © 2015 Po-Jung Huang et al. 2015 識別號： ISSN: 2314-6133 識別號： ISSN: 2314-6141 識別號： EISSN: 2314-6141 識別號： DOI: 10.1155/2015/409103 識別號： PMID: 25874210
顯示於類別:	[化學工程與材料工程學系 ] 期刊論文

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