Revisiting the streptavidin-biotin binding by using an aptamer and displacement isothermal calorimetry titration

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Title:	Revisiting the streptavidin-biotin binding by using an aptamer and displacement isothermal calorimetry titration
Authors:	陳文逸;Kuo, Tai-Chih;Tsai, Ching-Wei;Lee, Peng-Chen;Chen, Wen-Yih
Contributors:	工學院化學工程與材料工程學系
Keywords:	Affinity constant measurement;Aptamers, Nucleotide - metabolism;Binding;Biotin;Biotin - chemistry;Biotin - metabolism;Calorimetry;Calorimetry - methods;Constants;Entropy;ligand-displacement isothermal titration calorimetry;Ligands;Molecular interactions;Protein Binding;Streptavidin - chemistry;Streptavidin - metabolism;streptavidin-biotin binding;thermodynamic parameters;Thermodynamics;Titration;Titration calorimetry
Date:	2015-01-01
Issue Date:	2026-04-21 14:33:40 (UTC+8)
Publisher:	John Wiley and Sons Ltd;England: Blackwell Publishing Ltd
Abstract:	摘要： The association constant of a well‐known streptavidin–biotin binding has only been inferred from separately measured kinetic parameters. In a single experiment, we obtained Ka 1 × 1012 M−1 by using a streptavidin–binding aptamer and ligand‐displacement isothermal titration calorimetry. This study explores the challenges of determining thermodynamic parameters and the derived equilibrium binding affinity of tight ligand–receptor binding. Copyright © 2015 John Wiley & Sons, Ltd. For the first time, the complete thermodynamic parameters of the well‐known streptavidin (SA)‐biotin binding are obtained by using the ligand‐receptor isothermal titration calorimetry (LDITC) approaches, where the novel use of aptamers as the ligand analogs is proposed. The estimated Ka (1.0 × 1012 M−1) is slightly smaller than that derived from the separated kinetic studies of SA‐biotin binding. The large favorable enthalpy and entropy cost were obtained. This study demonstrates that aptamer and LDITC is a powerful tool for studying molecular interactions. 其他題名： J. Mol. Recognit 出版者： England: Blackwell Publishing Ltd 出版日期： 2015-03 出處： Journal of molecular recognition, 2015-03, Vol.28 (3), p.125-128 資源來源： Wiley Online Library - AutoHoldings Journals 版權： Copyright © 2015 John Wiley & Sons, Ltd. 識別號： ISSN: 0952-3499 識別號： ISSN: 1099-1352 識別號： EISSN: 1099-1352 識別號： DOI: 10.1002/jmr.2366 識別號： PMID: 25615849
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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