Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach: Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/101479

Title:	Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach: Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals
Authors:	謝發坤;Shieh, Fa-Kuen;Wang, Shao-Chun;Yen, Chia-I;Wu, Chang-Cheng;Dutta, Saikat;Chou, Lien-Yang;Morabito, Joseph V;Hu, Pan;Hsu, Ming-Hua;Wu, Kevin C.-W;Tsung, Chia-Kuang
Contributors:	理學院化學學系
Keywords:	Biocatalysis;biocatalysts;catalase;Catalase - chemistry;Catalase - metabolism;confocal microscopy;coordination polymers;crystals;electron microscopy;electrophoresis;Enzymes, Immobilized - chemistry;Enzymes, Immobilized - metabolism;hydrogen peroxide;Hydrogen Peroxide - metabolism;Imidazoles - chemistry;Kinetics;Models, Molecular;Nanopores;nitrogen;Organometallic Compounds - chemistry;Particle Size;Protein Conformation;proteinases;sorption;thermogravimetry;X-ray diffraction;Zeolites - chemistry
Date:	2015-04-08
Issue Date:	2026-04-21 14:35:15 (UTC+8)
Publisher:	American Chemical Society;United States: American Chemical Society
Abstract:	摘要： We develop a new concept to impart new functions to biocatalysts by combining enzymes and metal–organic frameworks (MOFs). The proof-of-concept design is demonstrated by embedding catalase molecules into uniformly sized ZIF-90 crystals via a de novo approach. We have carried out electron microscopy, X-ray diffraction, nitrogen sorption, electrophoresis, thermogravimetric analysis, and confocal microscopy to confirm that the ∼10 nm catalase molecules are embedded in 2 μm single-crystalline ZIF-90 crystals with ∼5 wt % loading. Because catalase is immobilized and sheltered by the ZIF-90 crystals, the composites show activity in hydrogen peroxide degradation even in the presence of protease proteinase K. 其他題名： J. Am. Chem. Soc 出版者： United States: American Chemical Society 出版日期： 2015-04-08 出處： Journal of the American Chemical Society, 2015-04, Vol.137 (13), p.4276-4279 資源來源： American Chemical Society Journals 版權： Copyright © American Chemical Society 識別號： ISSN: 0002-7863 識別號： ISSN: 1520-5126 識別號： EISSN: 1520-5126 識別號： DOI: 10.1021/ja513058h 識別號： PMID: 25781479
Appears in Collections:	[Department of Chemistry] journal & Dissertation

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