A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues

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Title:	A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
Authors:	樋口亞紺;Higuchi, Akon;Wang, Ching-Tang;Ling, Qing-Dong;Lee, Henry Hsin-chung;Kumar, S. Suresh;Chang, Yung;Alarfaj, Abdullah A.;Munusamy, Murugan A.;Hsu, Shih-Tien;Wu, Gwo-Jang;Umezawa, Akihiko
Contributors:	工學院化學工程與材料工程學系
Keywords:	13;13/107;631/61/2320;692/308/2171;96/100;Adipose Tissue - cytology;Adult Stem Cells - cytology;Adult Stem Cells - metabolism;Antigens, Surface - metabolism;Biomarkers;Cell culture;Cell Separation - methods;Gene Expression Profiling;Genotypes;Humanities and Social Sciences;Humans;Immunophenotyping;KLF4 protein;Membranes;Mesenchyme;multidisciplinary;Oct-4 protein;Pluripotency;Pore size;Purification;Science;Stem cells
Date:	2015-05-13
Issue Date:	2026-04-21 14:36:35 (UTC+8)
Publisher:	Nature Publishing Group;London: Nature Publishing Group UK
Abstract:	摘要： Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes ( Oct4 , Sox2 , Klf4 and Nanog ) compared with cells isolated using the conventional culture method. 其他題名： Sci Rep 出版者： London: Nature Publishing Group UK 出版日期： 2015-05-13 出處： Scientific reports, 2015-05, Vol.5 (1), p.10217, Article 10217 資源來源： ProQuest Open Access Content Collection 版權： The Author(s) 2015 版權： Copyright Nature Publishing Group May 2015 版權： Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited 識別號： ISSN: 2045-2322 識別號： EISSN: 2045-2322 識別號： DOI: 10.1038/srep10217 識別號： PMID: 25970301
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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