Differential dependencies on [Ca2+] and temperature of the monolayer spontaneous curvatures of DOPE, DOPA and cardiolipin: Effects of modulating the strength of the inter-headgroup repulsion

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題名:	Differential dependencies on [Ca2+] and temperature of the monolayer spontaneous curvatures of DOPE, DOPA and cardiolipin: Effects of modulating the strength of the inter-headgroup repulsion
作者:	陳儀帆;Chen, Y.-F;Tsang, K.-Y;Chang, W.-F;Fan, Z.-A
貢獻者:	工學院化學工程與材料工程學系
日期:	2015-05-28
上傳時間:	2026-04-21 14:37:04 (UTC+8)
出版者:	Royal Society of Chemistry
摘要:	摘要： Biomembranes assume nonlamellar structures in many cellular events, with the tendency of forming a nonlamellar structure quantified by the monolayer spontaneous curvature, C 0 , and with many of these events involving the acts of Ca 2+ . Despite this biologically important intimacy, how C 0 is affected by [Ca 2+ ] is unknown. In this study, we use the X-ray diffraction technique and the reconstruction of electron density profiles to measure the C 0 s of a zwitterionic phospholipid, DOPE, and two anionic phospholipids, DOPA and 18 : 1 (9 Z ) cardiolipin, at temperatures from 20 °C to 40 °C and [Ca 2+ ]s from 0 mM to 100 mM; these phospholipids are chosen to examine the contributions of the electric charge density per molecule. While showing a strong dependence on temperature, C 0,DOPE is nearly independent of [Ca 2+ ]. In contrast, C 0,DOPA and C 0,cardiolipin are almost unresponsive to the temperature change but affected by the [Ca 2+ ] variation; and C 0,DOPA varies with [Ca 2+ ] ∼1.5 times more strongly than C 0,cardiolipin , with the phase preferences of DOPA and cardiolipin shifting to the H II phase and remaining on the L α phase, respectively, at [Ca 2+ ] = 100 mM. From these observations, we reveal the effects of modulating the strength of the inter-headgroup repulsion and discuss the mechanisms underlying the phase behaviour and cellular functions of the investigated phospholipids. Most importantly, this study recognizes that the headgroup charge density is dominant in dictating the phase behaviour of the anionic phospholipids, and that the unique molecular characteristics of cardiolipin are critically needed both for maintaining the structural integrity of cardiolipin-rich biomembranes and for fulfilling the biological roles of the phospholipid. The measurements of spontaneous curvature for phospholipids differing in the headgroup charge density quantitatively and mechanistically reveal the importance of inter-headgroup repulsion to their phase behavior and cellular functions. 出版日期： 2015-05-13 識別號： ISSN: 1744-683X 識別號： EISSN: 1744-6848 識別號： DOI: 10.1039/c5sm00577a
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