Electrophoretic deposition to promote layer-by-layer assembly for in situ gene delivery application

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題名:	Electrophoretic deposition to promote layer-by-layer assembly for in situ gene delivery application
作者:	胡威文;Hu, Wei-Wen;Zheng, Yan-Rong
貢獻者:	工學院化學工程與材料工程學系
關鍵詞:	adsorption;Chitosan;colloids;contact angle;Deoxyribonucleic acid;Deposition;DNA;electric field;Electric fields;electrochemistry;electrodes;electrolytes;electrophoresis;Electrophoresis - methods;Electrophoretic deposition;gene expression;Genes;In situ transfection;in vitro studies;Layer-by-layer assembly;Multilayers;Polyelectrolytes;Polypyrrole;Surface chemistry;Transfection
日期:	2015-09-01
上傳時間:	2026-04-21 14:38:55 (UTC+8)
出版者:	Elsevier;Netherlands: Elsevier B.V
摘要:	摘要： •Electric field assistance (EFA) facilitates the assembly of DNA/chitosan multialyer.•DNA release is promoted as the electric field is only treated during DNA deposition.•Multilayers prepared by EFA at DNA deposition exhibit improved in situ transfection. In this study, layer-by-layer (LbL) assembly of polyelectrolyte was facilitated using electric field assistance (EFA). To elucidate the EFA effects on polyelectrolyte multilayers (PEMs), the electric fields were solely administrated to either chitosan or DNA adsorption. Both DNA and chitosan adsorptions can be augmented under low electric field due to the electrophoretic deposition. However, the ensuing electrochemical reactions on electrode interfered with interactions between multilayers when the bias was larger than 0.5V, leading to retard deposition. Subsequent delivery experiments indicated that EFA during DNA deposition demonstrated superior DNA release. In contrast, no obvious improvements were observed for the groups using the EFA during chitosan deposition. Moreover, water contact angle experiments revealed different effects of electric fields on multilayer structure. Using EFA during DNA deposition led to a layered-form composition, whereas interpenetration of electrolytes was enhanced with the application of the electric field during chitosan deposition. For in vitro experiments, EFA during DNA deposition significantly enhanced in situ transfection performances of PEMs that the transgene expression levels were increased and the periods were extended, suggesting this method is potential to quantitatively and temporally improve substrate-mediated gene delivery. 其他題名： Colloids Surf B Biointerfaces 出版者： Netherlands: Elsevier B.V 出版日期： 2015-09-01 出處： Colloids and surfaces, B, Biointerfaces, 2015-09, Vol.133, p.171-178 版權： 2015 Elsevier B.V. 版權： Copyright © 2015 Elsevier B.V. All rights reserved. 識別號： ISSN: 0927-7765 識別號： ISSN: 1873-4367 識別號： EISSN: 1873-4367 識別號： DOI: 10.1016/j.colsurfb.2015.05.046 識別號： PMID: 26101817
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