Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti

NCUIR > Engineering College > Department of Chemical and Materials Engineering > journal & Dissertation > Item 987654321/101622

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/101622

Title:	Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti
Authors:	樋口亞紺;Sujitha, Vasu;Murugan, Kadarkarai;Paulpandi, Manickam;Panneerselvam, Chellasamy;Suresh, Udaiyan;Roni, Mathath;Nicoletti, Marcello;Higuchi, Akon;Madhiyazhagan, Pari;Subramaniam, Jayapal;Dinesh, Devakumar;Vadivalagan, Chithravel;Chandramohan, Balamurugan;Alarfaj, Abdullah A.;Munusamy, Murugan A.;Barnard, Donald R.;Benelli, Giovanni
Contributors:	工學院化學工程與材料工程學系
Keywords:	Aedes - drug effects;Aedes aegypti;Animals;antiviral properties;Biomedical and Life Sciences;Biomedicine;Cercopithecus aethiops;Chemical synthesis;Control;control methods;dengue;Dengue - prevention & control;Dengue - virology;Dengue Virus;energy-dispersive X-ray analysis;Fourier transform infrared spectroscopy;Green Chemistry Technology;Health aspects;Humans;Immunology;Insecticides - pharmacology;instars;Larva - drug effects;larvae;lethal concentration 50;Medical Microbiology;Metal Nanoparticles - chemistry;Methods;Microbiology;Moringa oleifera;Moringa oleifera - chemistry;Mosquitoes;Nanoparticles;nanosilver;Original Paper;Plant Extracts - chemistry;Production processes;public health;pupae;scanning electron microscopy;seed extracts;Seeds - chemistry;serotypes;Silver;Silver - chemistry;Silver - pharmacology;Structure-Activity Relationship;structure-activity relationships;subtropics;vector control;Vero Cells;viral load;X-ray diffraction
Date:	2015-09-18
Issue Date:	2026-04-21 14:39:26 (UTC+8)
Publisher:	Springer Verlag;Berlin/Heidelberg: Springer Berlin Heidelberg
Abstract:	摘要： Dengue is an arthropod-borne viral infection mainly vectored through the bite of Aedes mosquitoes. Recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. There is no specific treatment for dengue. Its prevention and control solely depends on effective vector control measures. In this study, we proposed the green-synthesis of silver nanoparticles (AgNP) as a novel and effective tool against the dengue serotype DEN-2 and its major vector Aedes aegypti. AgNP were synthesized using the Moringa oleifera seed extract as reducing and stabilizing agent. AgNP were characterized using a variety of biophysical methods including UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and sorted for size categories. AgNP showed in vitro antiviral activity against DEN-2 infecting vero cells. Viral titer was 7 log₁₀ TCID₅₀/ml in control (AgNP-free), while it dropped to 3.2 log₁₀ TCID₅₀/ml after a single treatment with 20 μl/ml of AgNP. After 6 h, DEN-2 yield was 5.8 log₁₀ PFU/ml in the control, while it was 1.4 log₁₀ PFU/ml post-treatment with AgNP (20 μl/ml). AgNP were highly effective against the dengue vector A. aegypti, with LC₅₀ values ranging from 10.24 ppm (I instar larvae) to 21.17 ppm (pupae). Overall, this research highlighted the concrete potential of green-synthesized AgNP in the fight against dengue and its primary vector A. aegypti. Further research on structure–activity relationships of AgNP against other dengue serotypes is urgently required. 其他題名： Parasitol Res 出版者： Berlin/Heidelberg: Springer Berlin Heidelberg 出版日期： 2015-09-01 出處： Parasitology Research, 2015-09, Vol.114 (9), p.3315-3325 版權： Springer-Verlag Berlin Heidelberg 2015 版權： COPYRIGHT 2015 Springer 識別號： ISSN: 0932-0113 識別號： ISSN: 1432-1955 識別號： EISSN: 1432-1955 識別號： DOI: 10.1007/s00436-015-4556-2 識別號： PMID: 26063530
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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