Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces

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Title:	Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces
Authors:	樋口亞紺;Peng, I-Chia;Yeh, Chin-Chen;Lu, Yi-Tung;Muduli, Saradaprasan;Ling, Qing-Dong;Alarfaj, Abdullah A.;Munusamy, Murugan A.;Kumar, S. Suresh;Murugan, Kadarkarai;Lee, Hsin-chung;Chang, Yung;Higuchi, Akon
Contributors:	工學院化學工程與材料工程學系
Keywords:	Adipose Tissue - cytology;Advanced Basic Science;Biomaterial;Cell Adhesion;Cell culture;Coating;composite polymers;Continuous coating;Culture;culture dishes;Culture Media;cultured cells;Dentistry;Detaching;Detachment;Embryonic stem cell;embryonic stem cells;ethylene glycol;Humans;hydrophilicity;hydrophobicity;Nanobrush;Nanostructure;Nanotechnology;oligopeptides;polyacrylic acid;polyethylene glycol;Polystyrene resins;polystyrenes;Stem cell;Stem cells;Stem Cells - cytology;temperature;Thermoresponsive
Date:	2016-01-01
Issue Date:	2026-04-21 14:44:50 (UTC+8)
Publisher:	Elsevier BV;Netherlands: Elsevier Ltd
Abstract:	摘要： Stem cell culture is typically based on batch-type culture, which is laborious and expensive. Here, we propose a continuous harvest method for stem cells cultured on thermoresponsive nanobrush surfaces. In this method, stem cells are partially detached from the nanobrush surface by reducing the temperature of the culture medium below the critical solution temperature needed for thermoresponse. The detached stem cells are harvested by exchange into fresh culture medium. Following this, the remaining cells are continuously cultured by expansion in fresh culture medium at 37 °C. Thermoresponsive nanobrush surfaces were prepared by coating block copolymers containing polystyrene (for hydrophobic anchoring onto culture dishes) with three types of polymers: (a) polyacrylic acid with cell-binding oligopeptides, (b) thermoresponsive poly-N-isopropylacrylamide, and (c) hydrophilic poly(ethyleneglycol)methacrylate. The optimal coating durations and compositions for these copolymers to facilitate adequate attachment and detachment of human adipose-derived stem cells (hADSCs) and embryonic stem cells (hESCs) were determined. hADSCs and hESCs were continuously harvested for 5 and 3 cycles, respectively, via the partial detachment of cells from thermoresponsive nanobrush surfaces. 其他題名： Biomaterials 出版者： Netherlands: Elsevier Ltd 出版日期： 2016-01-01 出處： Biomaterials, 2016-01, Vol.76, p.76-86 版權： 2015 Elsevier Ltd 版權： Elsevier Ltd 版權： Copyright © 2015 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0142-9612 識別號： ISSN: 1878-5905 識別號： EISSN: 1878-5905 識別號： DOI: 10.1016/j.biomaterials.2015.10.039 識別號： PMID: 26519650
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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