Virus immobilization on biomaterial scaffolds through biotin-avidin interaction for improving bone regeneration

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題名:	Virus immobilization on biomaterial scaffolds through biotin-avidin interaction for improving bone regeneration
作者:	胡威文;Hu, Wei-Wen;Wang, Zhuo;Krebsbach, Paul H.
貢獻者:	工學院化學工程與材料工程學系
關鍵詞:	Adenoviridae - drug effects;Adenoviridae - metabolism;Animals;Avidin - metabolism;Biocompatible Materials - pharmacology;biomaterial scaffolds;Biotin - metabolism;biotin-avidin;bone regeneration;Bone Regeneration - drug effects;critical-sized bone defect;Gelatin - pharmacology;gene delivery;gene therapy;Gene Transfer Techniques;Genetic Vectors - metabolism;Mice;Osteogenesis - drug effects;Rats, Inbred F344;Regenerative medicine;Skull - diagnostic imaging;Skull - drug effects;Skull - pathology;Tissue Distribution - drug effects;Tissue engineering;Tissue Scaffolds - chemistry;Wound Healing - drug effects;X-Ray Microtomography
日期:	2016-02-01
上傳時間:	2026-04-21 14:48:36 (UTC+8)
出版者:	John Wiley and Sons Ltd;England: Blackwell Publishing Ltd
摘要:	摘要： To spatially control therapeutic gene delivery for potential tissue engineering applications, a biotin–avidin interaction strategy was applied to immobilize viral vectors on biomaterial scaffolds. Both adenoviral vectors and gelatin sponges were biotinylated and avidin was applied to link them in a virus–biotin–avidin–biotin–material (VBABM) arrangement. The tethered viral particles were stably maintained within scaffolds and SEM images illustrated that viral particles were evenly distributed in three‐dimensional (3D) gelatin sponges. An in vivo study demonstrated that transgene expression was restricted to the implant sites only and transduction efficiency was improved using this conjugation method. For an orthotopic bone regeneration model, adenovirus encoding BMP‐2 (AdBMP2) was immobilized to gelatin sponges before implanting into critical‐sized bone defects in rat calvaria. Compared to gelatin sponges with AdBMP2 loaded in a freely suspended form, the VBABM method enhanced gene transfer and bone regeneration was significantly improved. These results suggest that biotin–avidin immobilization of viral vectors to biomaterial scaffolds may be an effective strategy to facilitate tissue regeneration. Copyright © 2013 John Wiley & Sons, Ltd. 其他題名： J Tissue Eng Regen Med 出版者： England: Blackwell Publishing Ltd 出版日期： 2016-02 出處： Journal of tissue engineering and regenerative medicine, 2016-02, Vol.10 (2), p.E63-E72 版權： Copyright © 2013 John Wiley & Sons, Ltd. 版權： Copyright © 2016 John Wiley & Sons, Ltd. 識別號： ISSN: 1932-6254 識別號： ISSN: 1932-7005 識別號： EISSN: 1932-7005 識別號： DOI: 10.1002/term.1774 識別號： PMID: 23798490
顯示於類別:	[化學工程與材料工程學系 ] 期刊論文

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