A replaceable liposomal aptamer for the ultrasensitive and rapid detection of biotin

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题名:	A replaceable liposomal aptamer for the ultrasensitive and rapid detection of biotin
作者:	陳文逸;Sung, Tzu-Cheng;Chen, Wen-Yih;Shah, Pramod;Chen, Chien-Sheng
贡献者:	工學院化學工程與材料工程學系
关键词:	1,2-Dipalmitoylphosphatidylcholine - chemistry;631/92;639/638/11/872;9/10;Aptamers;Aptamers, Nucleotide - chemical synthesis;Aptamers, Nucleotide - chemistry;Binding sites;Binding, Competitive;Biosensing Techniques;Biotin;Biotin - analysis;Cholesterol;Cholesterol - chemistry;Competition;Humanities and Social Sciences;Limit of Detection;Lipids;Liposomes;Liposomes - chemistry;Multidisciplinary;Phosphatidylglycerols - chemistry;Polyethylene Glycols - chemistry;Science;Streptavidin;Streptavidin - chemistry
日期:	2016-02-23
上传时间:	2026-04-21 14:48:49 (UTC+8)
出版者:	Nature Publishing Group;London: Springer Science and Business Media LLC
摘要:	摘要： AbstractBiotin is an essential vitamin which plays an important role for maintaining normal physiological function. A rapid, sensitive and simple method is necessary to monitor the biotin level. Here, we reported a replacement assay for the detection of biotin using a replaceable liposomal aptamer. Replacement assay is a competitive assay where a sample analyte replaces the labeled competitor of analyte out of its biorecognition element on a surface. It is user friendly and time-saving because of washing free. We used aptamer as a competitor, not a biorecognition element as tradition. To label aptamers, we used cholesterol-conjugated aptamers to tag signal-amplifying-liposomes. Without the need of conjugation procedure, aptamers can be easily incorporated into the surface of dye-encapsulating liposomes. Two aptamers as competitors of biotin, ST-21 and ST-21M with different affinities to streptavidin, were studied in parallel for the detection of biotin using replacement assays. ST-21 and ST-21M aptamers reached to limits of detection of 1.32 pg/80 μl and 0.47 pg/80 μl, respectively. The dynamic ranges of our assays using ST-21 and ST-21M aptamers were seven and four orders of magnitude, respectively. This assay can be completed in 20 minutes without washing steps. These results were overall better than previous reported assays. 其他題名： Sci Rep 出版者： London: Springer Science and Business Media LLC 出版日期： 2016-02-23 出處： Scientific Reports, 2016-02, Vol.6 (1), p.21369-21369, Article 21369 資源來源： Springer Nature Link Open Access Journals 版權： The Author(s) 2016 版權： Copyright Nature Publishing Group Feb 2016 版權： Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited 識別號： ISSN: 2045-2322 識別號： EISSN: 2045-2322 識別號： DOI: 10.1038/srep21369 識別號： PMID: 26903199
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