資料載入中.....
|
請使用永久網址來引用或連結此文件:
https://ir.lib.ncu.edu.tw/handle/987654321/101950
|
| 題名: | Earthworm-mediated synthesis of silver nanoparticles: A potent tool against hepatocellular carcinoma, Plasmodium falciparum parasites and malaria mosquitoes |
| 作者: | 樋口亞紺;Jaganathan, Anitha;Murugan, Kadarkarai;Panneerselvam, Chellasamy;Madhiyazhagan, Pari;Dinesh, Devakumar;Vadivalagan, Chithravel;Aziz, Al Thabiani;Chandramohan, Balamurugan;Suresh, Udaiyan;Rajaganesh, Rajapandian;Subramaniam, Jayapal;Nicoletti, Marcello;Higuchi, Akon;Alarfaj, Abdullah A.;Munusamy, Murugan A.;Kumar, Suresh;Benelli, Giovanni |
| 貢獻者: | 工學院化學工程與材料工程學系 |
| 關鍵詞: | Animals;Anopheles - drug effects;Anopheles - parasitology;Anopheles stephensi;antibiotics;antiplasmodial properties;apoptosis;Arbovirus;Bacteria;Cancer;Carcinoma, Hepatocellular - drug therapy;Carcinoma, Hepatocellular - parasitology;cell proliferation;Chloroquine;cytotoxicity;dose response;drugs;earthworms;Eudrilus eugeniae;flow cytometry;Fourier transform infrared spectroscopy;Freshwater;fungi;Gambusia affinis;Gastroenterology and Hepatology;hepatoma;human cell lines;Humans;Infectious Disease;inhibitory concentration 50;insect vectors;Insect Vectors - drug effects;Insect Vectors - parasitology;instars;Larva;larvae;lethal concentration 50;Liver Neoplasms - drug therapy;Liver Neoplasms - parasitology;malaria;Malaria - drug therapy;Malaria - parasitology;Metal Nanoparticles - chemistry;Metal Nanoparticles - therapeutic use;moieties;nanoparticles;nanosilver;Nanosynthesis;natural enemies;neoplasm cells;Oligochaeta - chemistry;parasites;particle size;pathogens;Plasmodium falciparum;Plasmodium falciparum - drug effects;predation;Pupa;pupae;scanning electron microscopy;Silver - chemistry;Silver - pharmacology;Silver - therapeutic use;stabilizers |
| 日期: | 2016-06-01 |
| 上傳時間: | 2026-04-21 14:52:21 (UTC+8) |
| 出版者: | Elsevier Ireland Ltd;Netherlands: Elsevier Ireland Ltd |
| 摘要: | 摘要： The development of parasites and pathogens resistant to synthetic drugs highlighted the needing of novel, eco-friendly and effective control approaches. Recently, metal nanoparticles have been proposed as highly effective tools towards cancer cells and Plasmodium parasites. In this study, we synthesized silver nanoparticles (EW–AgNP) using Eudrilus eugeniae earthworms as reducing and stabilizing agents. EW–AgNP showed plasmon resonance reduction in UV–vis spectrophotometry, the functional groups involved in the reduction were studied by FTIR spectroscopy, while particle size and shape was analyzed by FESEM. The effect of EW–AgNP on in vitro HepG2 cell proliferation was measured using MTT assays. Apoptosis assessed by flow cytometry showed diminished endurance of HepG2 cells and cytotoxicity in a dose-dependent manner. EW–AgNP were toxic to Anopheles stephensi larvae and pupae, LC50 were 4.8ppm (I), 5.8ppm (II), 6.9ppm (III), 8.5ppm (IV), and 15.5ppm (pupae). The antiplasmodial activity of EW–AgNP was evaluated against CQ-resistant (CQ-r) and CQ-sensitive (CQ-s) strains of Plasmodium falciparum. EW–AgNP IC50 were 49.3μg/ml (CQ-s) and 55.5μg/ml (CQ-r), while chloroquine IC50 were 81.5μg/ml (CQ-s) and 86.5μg/ml (CQ-r). EW–AgNP showed a valuable antibiotic potential against important pathogenic bacteria and fungi. Concerning non-target effects of EW–AgNP against mosquito natural enemies, the predation efficiency of the mosquitofish Gambusia affinis towards the II and II instar larvae of A. stephensi was 68.50% (II) and 47.00% (III), respectively. In EW–AgNP-contaminated environments, predation was boosted to 89.25% (II) and 70.75% (III), respectively. Overall, this research highlighted the EW–AgNP potential against hepatocellular carcinoma, Plasmodium parasites and mosquito vectors, with little detrimental effects on mosquito natural enemies. [Display omitted] •Ag nanoparticles (AgNP) were synthesized using Eudrilus eugeniae earthworms.•AgNP were characterized by UV–vis spectrophotometry, FTIR spectroscopy and FESEM.•AgNP were spherical in shape, crystalline in nature, with a size range of 4–10nm.•Low doses of AgNP reduced in vitro HepG2 cell proliferation inducing apoptosis.•AgNP were toxic to chloroquine-resistant Plasmodium falciparum and its vector Anopheles stephensi.
其他題名： Parasitol Int
出版者： Netherlands: Elsevier Ireland Ltd
出版日期： 2016-06-01
出處： Parasitology International, 2016-06, Vol.65 (3), p.276-284
版權： 2016 Elsevier Ireland Ltd
版權： Elsevier Ireland Ltd
版權： Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
識別號： ISSN: 1383-5769
識別號： EISSN: 1873-0329
識別號： DOI: 10.1016/j.parint.2016.02.003
識別號： PMID: 26873539 |
| 顯示於類別: | [化學工程與材料工程學系 ] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| index.html | | 0Kb | HTML | 18 | 檢視/開啟 |
|
在NCUIR中所有的資料項目都受到原著作權保護.
|
