摘要： •5-DTAN was more effective than that of TAN on reducing skin tumorigenesis.•5-DTAN suppressed COX-2 protein expression more strongly than TAN.•5-DTAN promoted apoptosis in skin papillomas but not in TAN-treated groups. 5-Demethyltangeretin (5-DTAN), an autohydrolysis product of tangeretin (TAN) found in citrus peel, exhibited more potent anti-proliferative activity in human cancer cells than TAN itself. In this study, we investigated the anti-tumor promoting effect and underlying molecular mechanism of 5-DTAN on 7,12-dimethyl-benz(a)anthracene (DMBA)-induced and 12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted skin carcinogenesis. Application of 5-DTAN prior to each TPA-treatment was more effective than that of TAN on reducing the number, incidence and size of papillomas in DMBA-initiated mouse skin. Moreover, 5-DTAN suppressed cyclooxygenase-2 (COX-2) protein expression more strongly than TAN through interfering with phosphatidylinositiol 3-kinase (PI3K)/Akt signaling and further activation of transcription factor NF-κB. Taken together, these results revealed for the first time the in vivo chemopreventive efficacy of 5-DTAN on inhibition of skin carcinogenesis through promoting apoptosis and molecular interactions with residues of PI3K, COX-2, and AKT that may potentially serve as a novel functional agent capable of preventing inflammation-associated tumorigenesis. 出版者： Elsevier Ltd 出版日期： 2014-11-01 出處： Journal of functional foods, 2014-11, Vol.11, p.528-537 資源來源： Alma/SFX Local Collection 版權： 2014 Elsevier Ltd 識別號： ISSN: 1756-4646 識別號： ISSN: 2214-9414 識別號： EISSN: 2214-9414 識別號： DOI: 10.1016/j.jff.2014.08.009
