A chemical genetics approach reveals a role of brassinolide and cellulose synthase in hypocotyl elongation of etiolated Arabidopsis seedlings

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Title:	A chemical genetics approach reveals a role of brassinolide and cellulose synthase in hypocotyl elongation of etiolated Arabidopsis seedlings
Authors:	吳少傑;Chen, I.-Ju;Lo, Wan-Sheng;Chuang, Jung-Yun;Cheuh, Chiao-Mei;Fan, Yu-Shan;Lin, Lee-Chung;Wu, Shaw-Jye;Wang, Long-Chi
Contributors:	生醫理工學院生命科學系
Keywords:	1-aminocyclopropane-1-carboxylate synthase;ACC synthase;Acsinones;Amino Acids, Cyclic;Amino Acids, Cyclic - metabolism;Arabidopsis;Arabidopsis - genetics;Arabidopsis - growth & development;Arabidopsis - metabolism;Arabidopsis Proteins;Arabidopsis Proteins - genetics;Arabidopsis Proteins - metabolism;brassinolide;Brassinosteroids;Brassinosteroids - metabolism;cellulose;cellulose synthase;CESA6;Chemical genetics;chemical inhibitors;DET2;early development;ethylene;ethylene inhibitors;ethylene production;Ethylenes;Ethylenes - metabolism;etiolation;Etiolation - genetics;Etiolation - physiology;ETO1;Gene Expression Regulation, Plant;Genes, Plant;genetics;germination;Glucosyltransferases;Glucosyltransferases - genetics;Glucosyltransferases - metabolism;growth & development;Hypocotyl;Hypocotyl - growth & development;Hypocotyl - metabolism;hypocotyls;metabolism;molecular cloning;Mutation;pharmacology;Phenotype;physiology;Plant Development;Plant Development - genetics;Plant Development - physiology;Plant Growth Regulators;Plant Growth Regulators - genetics;Plant Growth Regulators - metabolism;plant reproduction;Plant Roots;Plant Roots - growth & development;Plant Roots - metabolism;Quinazolinones;Quinazolinones - metabolism;Quinazolinones - pharmacology;roots;seedling growth;seedlings;Seedlings - genetics;Seedlings - growth & development;Seedlings - metabolism;Steroids, Heterocyclic;Steroids, Heterocyclic - metabolism
Date:	2013-08-01
Issue Date:	2026-04-23 11:11:20 (UTC+8)
Publisher:	Elsevier Ireland Ltd;Ireland: Elsevier Ireland Ltd
Abstract:	摘要： •Use of chemical genetics to explore the roles of ethylene in seedling development.•Identification of ret mutants involved in hypocotyl elongation in etiolated seedlings.•Mutations in cellulose synthase6 and de-etiolated2 uncovered in 2 ret mutants.•Cellulose synthesis and brassinolides are required in regulating hypocotyl growth. The development of juvenile seedlings after germination is critical for the initial establishment of reproductive plants. Ethylene plays a pivotal role in the growth of seedlings under light or dark during early development. Previously, we identified small molecules sharing a quinazolinone backbone that suppressed the constitutive triple response phenotype in dark-grown eto1-4 seedlings. We designated these small molecules as ACSinhibitor quinazolinones (acsinones), which were uncompetitive inhibitors of 1-aminocyclopropane-1-carboxylic acid synthase. To explore the additional roles of acsinones in plants, we screened and identified 19 Arabidopsis mutants with reduced sensitivity to acsinone7303, which were collectively named revert to eto1 (ret) because of their recovery of the eto1 phenotype. A map-based cloning approach revealed that CELLULOSE SYNTHASE6 (CESA6) and DE-ETIOLATED2 (DET2) were mutated in ret8 (cesa6ret8;eto1-4) and ret41 (det2ret41;eto1-5), respectively. Etiolated seedlings of both ret8 and ret41 exhibit short hypocotyls and roots. Ethylene levels were similar in etiolated cesa6ret8 and det2-1 and in eto1 mutants treated with acsinone7303. Chemical inhibitors of ethylene biosynthesis and perception did not significantly suppress the etiolated phenotype of cesa6ret8 and det2ret41. However, together with eto1, cesa6ret8 and det2ret41 exhibited an enhanced phenotype in the hypocotyls and apical hooks of etiolated seedlings. These results confirm that, in addition to ethylene, cellulose synthesis and brassinolides can independently contribute to modulate hypocotyl development in young seedlings. 其他題名： Plant Sci 出版者： Ireland: Elsevier Ireland Ltd 出版日期： 2013-08-01 出處： Plant Science, 2013-08, Vol.209, p.46-57 版權： 2013 The Authors 版權： Copyright © 2013 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved. 識別號： ISSN: 0168-9452 識別號： ISSN: 1873-2259 識別號： EISSN: 1873-2259 識別號： DOI: 10.1016/j.plantsci.2013.04.005 識別號： PMID: 23759102
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