A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)

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Title:	A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)
Authors:	陸重安;Wu, Tsung-Meng;Lin, Ke-Chun;Liau, Wei-Shiang;Chao, Yun-Yang;Yang, Ling-Hung;Chen, Szu-Yun;Lu, Chung-An;Hong, Chwan-Yang
Contributors:	生醫理工學院生命科學系
Keywords:	ascorbate peroxidase;Ascorbate Peroxidases - genetics;Ascorbate Peroxidases - metabolism;Biochemistry;Biomarkers - metabolism;Biomedical and Life Sciences;fluorescence;fluorescent proteins;genes;Genes, Reporter;Genetic markers;Genetic Vectors;Green Fluorescent Proteins - genetics;Green Fluorescent Proteins - metabolism;Life Sciences;Luminescent Proteins - genetics;Luminescent Proteins - metabolism;Magnoliopsida;Mitochondria - metabolism;Molecular biology;Organelles - metabolism;Oryza - genetics;Oryza - metabolism;Oryza sativa;Plant biology;Plant Pathology;plant proteins;Plant Proteins - genetics;Plant Proteins - metabolism;Plant Sciences;Plants, Genetically Modified;protein tagging;Protein Transport;Proteins;Protoplasts;Recombinant Fusion Proteins;Rice;transgenic plants
Date:	2016-01-01
Issue Date:	2026-04-23 11:11:26 (UTC+8)
Publisher:	Springer Netherlands;Dordrecht: Springer Netherlands
Abstract:	摘要： In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins. 其他題名： Plant Mol Biol 出版者： Dordrecht: Springer Netherlands 出版日期： 2016-01-01 出處： Plant molecular biology, 2016-01, Vol.90 (1-2), p.107-115 版權： Springer Science+Business Media Dordrecht 2015 版權： Springer Science+Business Media Dordrecht 2016 識別號： ISSN: 0167-4412 識別號： EISSN: 1573-5028 識別號： DOI: 10.1007/s11103-015-0397-8 識別號： PMID: 26519260
Appears in Collections:	[Department of Life Science] journal & Dissertation

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