A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis

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Title:	A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis
Authors:	葉靖輝;Huang, Chun-Kai;Lo, Pei-Ching;Huang, Li-Fen;Wu, Shaw-Jye;Yeh, Ching-Hui;Lu, Chung-An
Contributors:	生醫理工學院生命科學系
Keywords:	abscisic acid;Arabidopsis;Arabidopsis - genetics;Arabidopsis - physiology;Arabidopsis Proteins - genetics;Arabidopsis Proteins - physiology;auxins;Biochemistry;Biomedical and Life Sciences;Chromatin Immunoprecipitation;Darkness;DNA-binding domains;Gene Expression Regulation, Plant;gene overexpression;genes;genetic markers;homeostasis;leaf development;Leaves;Life Sciences;mutants;phenotype;Plant biology;Plant Leaves - physiology;Plant Pathology;Plant Sciences;Plants;Plants, Genetically Modified;Real-Time Polymerase Chain Reaction;rice;sugars;transcription (genetics);transcription factors;Transcription Factors - genetics;Transcription Factors - physiology;transgenic plants
Date:	2015-06-26
Issue Date:	2026-04-23 11:11:29 (UTC+8)
Publisher:	Springer Netherlands;Dordrecht: Springer Netherlands
Abstract:	摘要： Leaf senescence, the final stage of leaf development, is regulated tightly by endogenous and environmental signals. MYBS3, a MYB transcription factor with a single DNA-binding domain, mediates sugar signaling in rice. Here we report that an Arabidopsis MYBS3 homolog, MYBH, plays a critical role in developmentally regulated and dark-induced leaf senescence by repressing transcription. Expression of MYBH was enhanced in older and dark-treated leaves. Gain- and loss-of-function analysis indicated that MYBH was involved in the onset of leaf senescence. Plants constitutively overexpressing MYBH underwent premature leaf senescence and showed enhanced expression of leaf senescence marker genes. In contrast, the MYBH mutant line, mybh - 1 , exhibited a delayed-senescence phenotype. The EAR repression domain was required for MYBH-regulated leaf senescence. Overexpression and knockout of MYBH repressed and enhanced auxin-responsive gene expression, respectively. MYBH repressed the auxin-amido synthase genes DFL1 / GH3.6 and DFL2 / GH3.10 , which regulate auxin homoeostasis, by binding directly to the TA box in each of their regulatory regions. An auxin-responsive phenotype was enhanced in MYBH overexpression lines and reduced in mybh knockout lines. Overexpression of MYBH enhanced gene expression of SAUR36 , an auxin-promoted leaf senescence key regulator, and accelerated ABA- and ethylene-induced leaf senescence in transgenic Arabidopsis plants. Our results suggest that the role of MYBH in controlling auxin homeostasis accounts for its capacity to participate in regulation of age- and darkness-induced leaf senescence in Arabidopsis. 其他題名： Plant Mol Biol 出版者： Dordrecht: Springer Netherlands 出版日期： 2015-06-01 出處： Plant molecular biology, 2015-06, Vol.88 (3), p.269-286 版權： Springer Science+Business Media Dordrecht 2015 識別號： ISSN: 0167-4412 識別號： ISSN: 1573-5028 識別號： EISSN: 1573-5028 識別號： DOI: 10.1007/s11103-015-0321-2 識別號： PMID: 25920996
Appears in Collections:	[Department of Life Science] journal & Dissertation

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