Androgen receptor inhibits epithelial-mesenchymal transition, migration, and invasion of PC-3 prostate cancer cells

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Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/102477

Title:	Androgen receptor inhibits epithelial-mesenchymal transition, migration, and invasion of PC-3 prostate cancer cells
Authors:	高永旭;Huo, Chieh;Kao, Yung-Hsi;Chuu, Chih-Pin
Contributors:	生醫理工學院生命科學系
Keywords:	Androgen Antagonists - pharmacology;Androgen receptor;Androgens;Anilides - pharmacology;Cell cycle;Cell Line, Tumor;Cell Movement;Epidermal Growth Factor - physiology;Epithelial-Mesenchymal Transition;Growth factors;Hematology, Oncology and Palliative Medicine;Humans;Immunoglobulins;Insulin-Like Growth Factor I - physiology;Invasion;Male;Matrix Metalloproteinase 2 - metabolism;Matrix Metalloproteinase 9 - metabolism;Metastasis;Microscopy;Migration;Neoplasm Invasiveness;Nitriles - pharmacology;PC-3;Prostate cancer;Prostatic Neoplasms - metabolism;Prostatic Neoplasms - pathology;Protein expression;Proteins;Receptors, Androgen - metabolism;Tosyl Compounds - pharmacology
Date:	2015-12-01
Issue Date:	2026-04-23 11:11:34 (UTC+8)
Publisher:	Elsevier Ireland Ltd;Ireland: Elsevier B.V
Abstract:	摘要： Bone metastasis is very common in prostate cancer (PCa) and causes severe pain. PC-3 is an androgen receptor (AR)-negative PCa cell line with high metastatic potential established from PCa bone metastasis. We observed that re-expression of AR, which is located in the cytoplasm in the absence of androgen, suppressed cell motility, migration, and invasion of PC-3 cells as determined by wound healing assay and transwell assay. Micro-Western Array and Western blotting analysis indicated that re-expression of AR increased APC, Akt2, Akt3, PI3K p85, phospho-PI3K p85 Tyr458, PI3K p85, and E-cadherin but decreased GSK-3β, phospho-GSK-3β Ser9, phospho-mTOR Ser2448, Skp2, NF-κB p50, Slug, N-cadherin, β-catenin, vimentin, MMP-9, and Snail. Migration and invasion of PC-3 and PC-3AR cells were promoted by EGF or IGF-1 but were suppressed by Casodex. Re-expression of AR reduced the activity of MMP-2 and MMP-9 in PC-3 cells. Our observations suggested that re-expressing AR suppresses migration and invasion of PC-3 cells via regulation of EMT marker proteins and MMP activity. 其他題名： Cancer Lett 出版者： Ireland: Elsevier B.V 出版日期： 2015-12-01 出處： Cancer letters, 2015-12, Vol.369 (1), p.103-111 版權： 2015 Elsevier Ireland Ltd 版權： Elsevier Ireland Ltd 版權： Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. 版權： Copyright Elsevier Limited Dec 1, 2015 識別號： ISSN: 0304-3835 識別號： ISSN: 1872-7980 識別號： EISSN: 1872-7980 識別號： DOI: 10.1016/j.canlet.2015.08.001 識別號： PMID: 26297988
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