Arabidopsis HIT4, a regulator involved in heat-triggered reorganization of chromatin and release of transcriptional gene silencing, relocates from chromocenters to the nucleolus in response to heat stress

NCU Institutional Repository > 生醫理工學院 > 生命科學系 > 期刊論文 > Item 987654321/102487

請使用永久網址來引用或連結此文件: https://ir.lib.ncu.edu.tw/handle/987654321/102487

題名:	Arabidopsis HIT4, a regulator involved in heat-triggered reorganization of chromatin and release of transcriptional gene silencing, relocates from chromocenters to the nucleolus in response to heat stress
作者:	吳家榮;Wang, Lian‐Chin;Wu, Jia‐Rong;Hsu, Yi‐Ju;Wu, Shaw‐Jye
貢獻者:	生醫理工學院生命科學系
關鍵詞:	Arabidopsis;Arabidopsis - genetics;Arabidopsis - metabolism;Arabidopsis Proteins - analysis;Arabidopsis Proteins - genetics;Arabidopsis Proteins - metabolism;Arabidopsis thaliana;cell nucleolus;Cell Nucleolus - metabolism;Chromatin;chromatin reorganization;chromocenter decondensation;chromocenters;Deoxyribonucleic acid;DNA;DNA methylation;Fluorescence;fluorescent proteins;Gene Expression Regulation, Plant;Gene Silencing;Green Fluorescent Proteins - analysis;Heat;Heat stress;Heat tolerance;Heat-Shock Response;HEAT‐INTOLERANT 4 (HIT4);Hypersensitivity;MORPHEUS' MOLECULE 1 (MOM1);Morphology;mutants;Nuclear Proteins - analysis;Nuclear Proteins - metabolism;Nucleoli;Plants, Genetically Modified;Protein Transport;Regulatory sequences;Relocation;transcription (genetics);Transcription Factors - analysis;Transcription Factors - genetics;Transcription Factors - metabolism;transcriptional gene silencing
日期:	2015-01-01
上傳時間:	2026-04-23 11:11:43 (UTC+8)
出版者:	Wiley-Blackwell Publishing Ltd;England: Academic Press
摘要:	摘要： Arabidopsis HIT4 is known to mediate heat‐induced decondensation of chromocenters and release from transcriptional gene silencing (TGS) with no change in the level of DNA methylation. It is unclear whether HIT4 and MOM1, a well‐known DNA methylation‐independent transcriptional silencer, have overlapping regulatory functions. A hit4‐1/mom1 double mutant strain was generated. Its nuclear morphology and TGS state were compared with those of wild‐type, hit4‐1, and mom1 plants. Fluorescent protein tagging was employed to track the fates of HIT4, hit4‐1 and MOM1 in vivo under heat stress. HIT4‐ and MOM1‐mediated TGS were distinguishable. Both HIT4 and MOM1 were localized normally to chromocenters. Under heat stress, HIT4 relocated to the nucleolus, whereas MOM1 dispersed with the chromocenters. hit4‐1 was able to relocate to the nucleolus under heat stress, but its relocation was insufficient to trigger the decompaction of chromocenters. The hypersensitivity to heat associated with the impaired reactivation of TGS in hit4‐1 was not alleviated by mom1‐induced release from TGS. HIT4 delineates a novel and MOM1‐independent TGS regulation pathway. The involvement of a currently unidentified component that links HIT4 relocation and the large‐scale reorganization of chromatin, and which is essential for heat tolerance in plants is hypothesized. 其他題名： New Phytol 出版者： England: Academic Press 出版日期： 2015 出處： The New phytologist, 2015, Vol.205 (2), p.544-554 資源來源： Wiley Online Library Journals 版權： 2015 New Phytologist Trust 版權： 2014 The Authors. New Phytologist © 2014 New Phytologist Trust 版權： 2014 The Authors. New Phytologist © 2014 New Phytologist Trust. 版權： Copyright © 2014 New Phytologist Trust 識別號： ISSN: 0028-646X 識別號： ISSN: 1469-8137 識別號： EISSN: 1469-8137 識別號： DOI: 10.1111/nph.13088 識別號： PMID: 25329561
顯示於類別:	[生命科學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	17	檢視/開啟

在NCUIR中所有的資料項目都受到原著作權保護.

社群 sharing

資料載入中.....