Arabidopsis mTERF15 is required for mitochondrial nad2 intron 3 splicing and functional complex I activity

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题名:	Arabidopsis mTERF15 is required for mitochondrial nad2 intron 3 splicing and functional complex I activity
作者:	謝明勳;Hsu, Ya-Wen;Wang, Huei-Jing;Hsieh, Ming-Hsiun;Hsieh, Hsu-Liang;Jauh, Guang-Yuh
贡献者:	生醫理工學院生命科學系
关键词:	Arabidopsis;Arabidopsis - genetics;Arabidopsis - growth & development;Arabidopsis - metabolism;Arabidopsis Proteins - genetics;Arabidopsis Proteins - metabolism;Arabidopsis thaliana;Binding;Biology and life sciences;Biosynthesis;Chloroplasts;Cytosol;Defects;Deoxyribonucleic acid;DNA;DNA biosynthesis;DNA methylation;DNA replication;Electron transport;Electron transport chain;Electron Transport Complex I - metabolism;Evolution;Gene expression;Genes;Genetics;Genomes;Genomics;Intermediates;Introns;Membrane Potential, Mitochondrial;Metabolism;Mitochondria;Mitochondria - genetics;Mitochondria - metabolism;Mitochondria - ultrastructure;Mitochondrial DNA;Mitochondrial Turnover - genetics;Mutation;Nuclei;Nuclei (cytology);Nucleotide sequence;Phenotype;Physiological aspects;Plant biology;Plant growth;Plant mitochondria;Plastids;Protein Transport;Proteins;Respiration;Ribonucleic acid;RNA;RNA Splicing;Splicing factors;Structural integrity;T-DNA;Termination factors;Transcription factors;Transcription initiation;Transcription termination
日期:	2014-11-17
上传时间:	2026-04-23 11:11:44 (UTC+8)
出版者:	Public Library of Science;United States: Public Library of Science (PLoS)
摘要:	摘要： Mitochondria play a pivotal role in most eukaryotic cells, as they are responsible for the generation of energy and diverse metabolic intermediates for many cellular events. During endosymbiosis, approximately 99% of the genes encoded by the mitochondrial genome were transferred into the host nucleus, and mitochondria import more than 1000 nuclear-encoded proteins from the cytosol to maintain structural integrity and fundamental functions, including DNA replication, mRNA transcription and RNA metabolism of dozens of mitochondrial genes. In metazoans, a family of nuclear-encoded proteins called the mitochondrial transcription termination factors (mTERFs) regulates mitochondrial transcription, including transcriptional termination and initiation, via their DNA-binding activities, and the dysfunction of individual mTERF members causes severe developmental defects. Arabidopsis thaliana and Oryza sativa contain 35 and 48 mTERFs, respectively, but the biological functions of only a few of these proteins have been explored. Here, we investigated the biological role and molecular mechanism of Arabidopsis mTERF15 in plant organelle metabolism using molecular genetics, cytological and biochemical approaches. The null homozygous T-DNA mutant of mTERF15, mterf15, was found to result in substantial retardation of both vegetative and reproductive development, which was fully complemented by the wild-type genomic sequence. Surprisingly, mitochondria-localized mTERF15 lacks obvious DNA-binding activity but processes mitochondrial nad2 intron 3 splicing through its RNA-binding ability. Impairment of this splicing event not only disrupted mitochondrial structure but also abolished the activity of mitochondrial respiratory chain complex I. These effects are in agreement with the severe phenotype of the mterf15 homozygous mutant. Our study suggests that Arabidopsis mTERF15 functions as a splicing factor for nad2 intron 3 splicing in mitochondria, which is essential for normal plant growth and development. 其他題名： PLoS One 出版者： United States: Public Library of Science (PLoS) 出版日期： 2014-11-17 出處： PLoS ONE, 2014-11, Vol.9 (11), p.e 資源來源： Agricultural & Environmental Science Collection 版權： COPYRIGHT 2014 Public Library of Science 版權： 2014 Hsu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2014 Hsu et al 2014 Hsu et al 識別號： ISSN: 1932-6203 識別號： EISSN: 1932-6203 識別號： DOI: 10.1371/journal.pone.0112360 識別號： PMID: 25402171
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