Biotransformation of trinitrotoluene (TNT) by Pseudomonas spp. isolated from a TNT-contaminated environment

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题名:	Biotransformation of trinitrotoluene (TNT) by Pseudomonas spp. isolated from a TNT-contaminated environment
作者:	陳師慶;Chien, Chih‐Ching;Kao, Chih‐Ming;Chen, De‐Yu;Chen, Ssu Ching;Chen, Chien‐Cheng
贡献者:	生醫理工學院生命科學系
关键词:	Applied sciences;Bacteria;Base Sequence;Biodegradation;Biodegradation of pollutants;Biodegradation, Environmental;Biological and medical sciences;Biological and physicochemical phenomena;Biotechnology;Biotransformation;Carbon;Chromatography;Contamination;culture media;Culture techniques;Decontamination. Miscellaneous;Degradation;Energy sources;enrichment culture;Environment and pollution;Exact sciences and technology;Explosive Agents;Explosive Agents - metabolism;Fundamental and applied biological sciences. Psychology;genes;genetics;Groundwater;growth & development;high performance liquid chromatography;Industrial applications and implications. Economical aspects;isolation & purification;Liquid chromatography;metabolism;Molecular Sequence Data;Natural water pollution;Nitrogen;Pollutants;polluted soils;Pollution;Pseudomonas;Pseudomonas aeruginosa;Pseudomonas aeruginosa - genetics;Pseudomonas aeruginosa - growth & development;Pseudomonas aeruginosa - isolation & purification;Pseudomonas aeruginosa - metabolism;Pseudomonas putida;Pseudomonas putida - genetics;Pseudomonas putida - growth & development;Pseudomonas putida - isolation & purification;Pseudomonas putida - metabolism;Pseudomonas sp;Ribonucleic acid;ribosomal RNA;RNA;Soil (material);Soil and sediments pollution;Soil contamination;Soil Microbiology;Soil Pollutants;Soil Pollutants - metabolism;Strain;TNT;Trinitrotoluene;Trinitrotoluene - metabolism;Water treatment and pollution
日期:	2014-01-01
上传时间:	2026-04-23 11:11:50 (UTC+8)
出版者:	Wiley-Blackwell;Pensacola, FL: Blackwell Publishing Ltd
摘要:	摘要： The compound 2,4,6‐trinitrotoluene (TNT) is a secondary explosive widely used worldwide for both military and civil purposes. As a result, residual TNT has been detected as an environmental pollutant in both soil and groundwater. The authors have isolated several microbial strains from soil contaminated with TNT by enrichment culture techniques using TNT as a carbon, nitrogen, and energy source. The contaminated soil contained approximately 1860 ppm TNT measured by high‐performance liquid chromatography (HPLC). The initial identification of these isolates was determined by 16S rRNA gene comparison. The isolates mainly included species belonging to the genus Pseudomonas. Two strains (Pseudomonas putida strain TP1 and Pseudomonas aeruginosa strain TP6) were selected for further examination. Both strains demonstrated the ability to grow on the medium containing TNT as a carbon, energy, and nitrogen source and also clearly demonstrated the ability to degrade TNT. More than 90% of the TNT in the growth medium was degraded by both strains after 22 d incubation, as determined by HPLC. Additionally, the resting cells of P. putida TP1 and P. aeruginosa TP6 both significantly displayed the ability to transform (metabolize) TNT. Environ Toxicol Chem 2014;33:1059–1063. © 2014 SETAC 其他題名： Environ Toxicol Chem 出版者： Pensacola, FL: Blackwell Publishing Ltd 出版日期： 2014-05 出處： Environmental toxicology and chemistry, 2014-05, Vol.33 (5), p.1059-1063 資源來源： Wiley Online Library - AutoHoldings Journals 版權： 2014 SETAC 版權： 2015 INIST-CNRS 版權： 2014 SETAC. 版權： Copyright Blackwell Publishing Ltd. May 2014 識別號： ISSN: 0730-7268 識別號： ISSN: 1552-8618 識別號： EISSN: 1552-8618 識別號： DOI: 10.1002/etc.2553 識別號： PMID: 24549634 識別號： CODEN: ETOCDK
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