Calreticulin activates β1 integrin via fucosylation by fucosyltransferase 1 in J82 human bladder cancer cells

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Title:	Calreticulin activates β1 integrin via fucosylation by fucosyltransferase 1 in J82 human bladder cancer cells
Authors:	吳沛翊;Lu, Yi-Chien;Chen, Chiung-Nien;Chu, Chia-Ying;Lu, JenHer;Wang, Bo-Jeng;Chen, Chia-Hua;Huang, Min-Chuan;Lin, Tsui-Hwa;Pan, Chin-Chen;Chen, Swey-Shen Alex;Hsu, Wen-Ming;Liao, Yung-Feng;Wu, Pei-Yi;Hsia, Hsin-Yi;Chang, Cheng-Chi;Lee, Hsinyu
Contributors:	生醫理工學院生命科學系
Keywords:	Calreticulin - biosynthesis;Cell Adhesion - genetics;Cell Line, Tumor;Fucosyltransferases - genetics;Fucosyltransferases - physiology;Galactoside 2-alpha-L-fucosyltransferase;Humans;Integrin beta1 - genetics;Integrin beta1 - metabolism;Protein Stability;RNA Stability - genetics;Urinary Bladder Neoplasms - genetics;Urinary Bladder Neoplasms - metabolism;Urinary Bladder Neoplasms - pathology
Date:	2014-05-15
Issue Date:	2026-04-23 11:11:55 (UTC+8)
Publisher:	Portland Press, Ltd.;England: Portland Press Ltd
Abstract:	摘要： Fucosylation regulates various pathological events in cells. We reported that different levels of CRT (calreticulin) affect the cell adhesion and metastasis of bladder cancer. However, the precise mechanism of tumour metastasis regulated by CRT remains unclear. Using a DNA array, we identified FUT1 (fucosyltransferase 1) as a gene regulated by CRT expression levels. CRT regulated cell adhesion through α1,2-linked fucosylation of β1 integrin and this modification was catalysed by FUT1. To clarify the roles for FUT1 in bladder cancer, we transfected the human FUT1 gene into CRT-RNAi stable cell lines. FUT1 overexpression in CRT-RNAi cells resulted in increased levels of β1 integrin fucosylation and rescued cell adhesion to type-I collagen. Treatment with UEA-1 (Ulex europaeus agglutinin-1), a lectin that recognizes FUT1-modified glycosylation structures, did not affect cell adhesion. In contrast, a FUT1-specific fucosidase diminished the activation of β1 integrin. These results indicated that α1,2-fucosylation of β1 integrin was not involved in integrin–collagen interaction, but promoted β1 integrin activation. Moreover, we demonstrated that CRT regulated FUT1 mRNA degradation at the 3′-UTR. In conclusion, the results of the present study suggest that CRT stabilized FUT1 mRNA, thereby leading to an increase in fucosylation of β1 integrin. Furthermore, increased fucosylation levels activate β1 integrin, rather than directly modifying the integrin-binding sites. 其他題名： Biochem J 出版者： England: Portland Press Ltd 出版日期： 2014-05-15 出處： Biochemical Journal, 2014-05, Vol.460 (1), p.69-80 識別號： ISSN: 0264-6021 識別號： ISSN: 1470-8728 識別號： EISSN: 1470-8728 識別號： DOI: 10.1042/bj20131424 識別號： PMID: 24593306
Appears in Collections:	[Department of Life Science] journal & Dissertation

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