Circadian phase-dependent effect of nitric oxide on L-type voltage-gated calcium channels in avian cone photoreceptors

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Title:	Circadian phase-dependent effect of nitric oxide on L-type voltage-gated calcium channels in avian cone photoreceptors
Authors:	黃佳瑜;Ko, Michael L.;Shi, Liheng;Huang, Cathy C.‐Y.;Grushin, Kirill;Park, So‐Young;Ko, Gladys Y.‐P.
Contributors:	生醫理工學院生命科學系
Keywords:	AKT protein;Animals;Birds;Blotting, Western;Brain;Calcium Channels, L-Type - drug effects;Chick Embryo;circadian;Circadian rhythm;Circadian Rhythm - physiology;cone;Cyclic GMP - physiology;Cyclic GMP-Dependent Protein Kinases - metabolism;Immunoenzyme Techniques;In Vitro Techniques;ion channel;Kinases;MAP Kinase Signaling System - drug effects;MAP Kinase Signaling System - physiology;Neurochemistry;Nitrates - metabolism;Nitric oxide;Nitric Oxide - pharmacology;Nitric Oxide Donors - pharmacology;Nitric Oxide Synthase - metabolism;Oncogene Protein v-akt - physiology;Patch-Clamp Techniques;Phosphatidylinositol 3-Kinases - physiology;photoreceptor;retina;Retinal Cone Photoreceptor Cells - drug effects;RNA, Small Interfering - genetics;S-Nitroso-N-Acetylpenicillamine - pharmacology;Signal Transduction - drug effects;signaling;Transfection
Date:	2013-11-01
Issue Date:	2026-04-23 11:12:08 (UTC+8)
Publisher:	Wiley-Blackwell Publishing Ltd;England: Blackwell Publishing Ltd
Abstract:	摘要： Nitric oxide (NO) plays an important role in phase‐shifting of circadian neuronal activities in the suprachiasmatic nucleus and circadian behavior activity rhythms. In the retina, NO production is increased in a light‐dependent manner. While endogenous circadian oscillators in retinal photoreceptors regulate their physiological states, it is not clear whether NO also participates in the circadian regulation of photoreceptors. In this study, we demonstrate that NO is involved in the circadian phase‐dependent regulation of L‐type voltage‐gated calcium channels (L‐VGCCs). In chick cone photoreceptors, the L‐VGCCα1 subunit expression and the maximal L‐VGCC currents are higher at night, and both Ras‐mitogen‐activated protein kinase (MAPK)‐extracellular signal‐regulated kinase (Erk) and Ras‐phosphatidylinositol 3 kinase (PI3K)‐protein kinase B (Akt) are part of the circadian output pathways regulating L‐VGCCs. The NO‐cGMP‐protein kinase G (PKG) pathway decreases L‐VGCCα1 subunit expression and L‐VGCC currents at night, but not during the day, and exogenous NO donor or cGMP decreases the phosphorylation of Erk and Akt at night. The protein expression of neural NO synthase (nNOS) is also under circadian control, with both nNOS and NO production being higher during the day. Taken together, NO/cGMP/PKG signaling is involved as part of the circadian output pathway to regulate L‐VGCCs in cone photoreceptors. In cone photoreceptors, the protein expression of neural nitric oxide synthase (nNOS) and NO production are under circadian control. NO‐cGMP‐protein kinase G (PKG) signaling serves in the circadian output pathway to regulate the circadian rhythms of L‐type voltage‐gated calcium channels (L‐VGCCs) in part through regulating the phosphorylation states of extracellular‐signal‐regulated kinase (Erk) and protein kinase B (Akt). In cone photoreceptors, the protein expression of neural nitric oxide synthase (nNOS) and NO production are under circadian control. NO‐cGMP‐protein kinase G (PKG) signaling serves in the circadian output pathway to regulate the circadian rhythms of L‐type voltage‐gated calcium channels (L‐VGCCs) in part through regulating the phosphorylation states of extracellular‐signal‐regulated kinase (Erk) and protein kinase B (Akt). 其他題名： J Neurochem 出版者： England: Blackwell Publishing Ltd 出版日期： 2013-11 出處： Journal of neurochemistry, 2013-11, Vol.127 (3), p.314-328 資源來源： Wiley Online Library 版權： 2013 International Society for Neurochemistry 版權： 2013 International Society for Neurochemistry. 版權： Copyright © 2013 International Society for Neurochemistry 識別號： ISSN: 0022-3042 識別號： ISSN: 1471-4159 識別號： EISSN: 1471-4159 識別號： DOI: 10.1111/jnc.12384 識別號： PMID: 23895452
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