Curcumin Sensitizes Hepatocellular Carcinoma Cells to Radiation via Suppression of Radiation-Induced NF-κB Activity

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Title:	Curcumin Sensitizes Hepatocellular Carcinoma Cells to Radiation via Suppression of Radiation-Induced NF-κB Activity
Authors:	許斐婷;Hwang, Jeng-Jong;Liu, Tsu-Te;Liu, Yu-Chang;Hsu, Fei-Ting
Contributors:	生醫理工學院生命科學系
Keywords:	Animals;Apoptosis - drug effects;Apoptosis - radiation effects;Cancer cells;Carcinoma, Hepatocellular - drug therapy;Carcinoma, Hepatocellular - genetics;Carcinoma, Hepatocellular - pathology;Carcinoma, Hepatocellular - radiotherapy;Cell Proliferation - drug effects;Cell Proliferation - radiation effects;Curcumin - administration & dosage;Drug therapy;Gene Expression Regulation, Neoplastic - drug effects;Gene Expression Regulation, Neoplastic - radiation effects;Health aspects;Hep G2 Cells;Hepatoma;Humans;I-kappa B Proteins - genetics;Liver Neoplasms - drug therapy;Liver Neoplasms - genetics;Liver Neoplasms - pathology;Liver Neoplasms - radiotherapy;Membrane Potential, Mitochondrial - drug effects;Membrane Potential, Mitochondrial - radiation effects;Methods;Mice;NF-kappa B - biosynthesis;NF-kappa B - genetics;NF-KappaB Inhibitor alpha;Radiotherapy;Turmeric;Xenograft Model Antitumor Assays
Date:	2015-01-01
Issue Date:	2026-04-23 11:12:13 (UTC+8)
Publisher:	Hindawi Publishing Corporation;Cairo, Egypt: Hindawi Publishing Corporation
Abstract:	摘要： The effects and possible underlying mechanism of curcumin combined with radiation in human hepatocellular carcinoma (HCC) cells in vitro were evaluated. The effects of curcumin, radiation, and combination of both on cell viability, apoptosis, NF-κB activation, and expressions of NF-κB downstream effector proteins were investigated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), NF-κB reporter gene, mitochondrial membrane potential (MMP), electrophoretic mobility shift (EMSA), and Western blot assays in Huh7-NF-κB-luc2, Hep3B, and HepG2 cells. Effect of I kappa B alpha mutant (IκBαM) vector, a specific inhibitor of NF-κB activation, on radiation-induced loss of MMP was also evaluated. Results show that curcumin not only significantly enhances radiation-induced cytotoxicity and depletion of MMP but inhibits radiation-induced NF-κB activity and expressions of NF-κB downstream proteins in HCC cells. IκBαM vector also shows similar effects. In conclusion, we suggest that curcumin augments anticancer effects of radiation via the suppression of NF-κB activation. 其他題名： Biomed Res Int 出版者： Cairo, Egypt: Hindawi Publishing Corporation 出版日期： 2015-01-01 出處： BioMed research international, 2015-01, Vol.2015 (2015), p.1-7 資源來源： Academic Search Premier (Ebsco) 版權： Copyright © 2015 Fei-Ting Hsu et al. 版權： COPYRIGHT 2015 John Wiley & Sons, Inc. 版權： Copyright © 2015 Fei-Ting Hsu et al. 2015 識別號： ISSN: 2314-6133 識別號： ISSN: 2314-6141 識別號： EISSN: 2314-6141 識別號： DOI: 10.1155/2015/363671 識別號： PMID: 26539482
Appears in Collections:	[Department of Life Science] journal & Dissertation

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