Cyclooxygenase-2 expression is up-regulated by 2-aminobiphenyl in a ROS and MAPK-dependent signaling pathway in a bladder cancer cell line

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题名:	Cyclooxygenase-2 expression is up-regulated by 2-aminobiphenyl in a ROS and MAPK-dependent signaling pathway in a bladder cancer cell line
作者:	陳師慶;Chen, Chien-Cheng;Cheng, Yu-Yang;Chen, Ssu-Ching;Tuan, Yen-Fan;Chen, Yun-Ju;Chen, Chien-Yen;Chen, Lei-Chin
贡献者:	生醫理工學院生命科學系
关键词:	Aminobiphenyl Compounds - toxicity;Cell Line, Tumor;Cell Survival - drug effects;Cyclooxygenase 2 - genetics;Gene Expression Regulation, Enzymologic - drug effects;Hair Dyes;Humans;MAP Kinase Signaling System - drug effects;NADPH Oxidases - metabolism;Reactive Oxygen Species - metabolism;Tobacco Smoke Pollution;Up-Regulation;Urinary Bladder Neoplasms
日期:	2012-03-19
上传时间:	2026-04-23 11:12:16 (UTC+8)
出版者:	United States: American Chemical Society
摘要:	摘要： Overexposure to biphenyl amine compounds, which are found in smoke and azo-dyes, is linked to the occurrence of bladder cancer. However, the molecular mechanisms of biphenyl amine compound-induced bladder cancer are still unclear. Many studies have demonstrated that overexpression of cyclooxygenase-2 (COX-2) in neoplastic lesions is associated with carcinogenesis. In this study, we have demonstrated that 2-aminobiphenyl (2-ABP) up-regulated the expression of COX-2 in a dose- and time-dependent manner in TSGH-8301 bladder cancer cells. This 2-ABP-induced COX-2 expression was attenuated by ROS scavenger NAC and NADPH oxidase inhibitors apocynin and DPI. The p22phox subunit of NADPH oxidase, but not p67, and Nox2 was up-regulated by 2-ABP. Knocking down p22phox by siRNA significantly reduced 2-ABP-induced COX-2 expression. Furthermore, 2-ABP also activated the ERK/JNK-AP1 pathways, and this effect was also abolished by NADPH oxidase inhibitors. Blocking the ERK/JNK-AP1 signaling pathways by pharmacological inhibitors attenuated 2-ABP-induced COX-2 expression. Overexpression of the upstream ERK activator MEK1 significantly and consistently increased 2-ABP-mediated COX-2 expression. Transfection of a dominant negative c-Jun mutant, TAM-67, blocked 2-ABP-mediated COX-2 expression, demonstrating that c-Jun was responsible for the transcriptional activation. Taken together, these results demonstrate that 2-ABP induces the carcinogenic factor COX-2 and that this induction is mediated through NADPH oxidase-derived ROS-dependent JNK/ERK-AP-1 pathways. 其他題名： Chem. Res. Toxicol 出版者： United States: American Chemical Society 出版日期： 2012-03-19 出處： Chemical Research in Toxicology, 2012-03, Vol.25 (3), p.695-705 資源來源： American Chemical Society Journals 版權： Copyright © 2012 American Chemical Society 版權： 2012 American Chemical Society 識別號： ISSN: 0893-228X 識別號： ISSN: 1520-5010 識別號： EISSN: 1520-5010 識別號： DOI: 10.1021/tx2004689 識別號： PMID: 22288910
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