Differential regulation of Arabidopsis plastid gene expression and RNA editing in non-photosynthetic tissues

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Title:	Differential regulation of Arabidopsis plastid gene expression and RNA editing in non-photosynthetic tissues
Authors:	謝明勳;Tseng, Ching-Chih;Lee, Chih-Jen;Chung, Yi-Ting;Sung, Tzu-Ying;Hsieh, Ming-Hsiun
Contributors:	生醫理工學院生命科學系
Keywords:	albino;Arabidopsis;Arabidopsis - genetics;Arabidopsis - metabolism;Arabidopsis Proteins;Arabidopsis Proteins - genetics;Biochemistry;Biomedical and Life Sciences;Chloroplast Proteins;Chloroplast Proteins - genetics;Cytochrome b Group;Cytochrome b Group - genetics;DNA-directed RNA polymerase;drug effects;etiolation;Gene expression;Gene Expression Regulation, Plant;Gene Expression Regulation, Plant - drug effects;Gene Expression Regulation, Plant - genetics;genes;genetics;Life Sciences;lincomycin;Lincomycin - pharmacology;metabolism;mitochondria;mutants;NADH Dehydrogenase;NADH Dehydrogenase - genetics;pharmacology;Photosystem II Protein Complex;Photosystem II Protein Complex - genetics;physiology;Plant biology;Plant Pathology;Plant Sciences;Plastids;Plastids - genetics;Plastids - metabolism;Ribonucleic acid;RNA;RNA editing;RNA Editing - genetics;RNA Editing - physiology;RNA, Plant;RNA, Plant - genetics;roots;Seedlings;Tissues
Date:	2013-07-01
Issue Date:	2026-04-23 11:12:18 (UTC+8)
Publisher:	Springer Netherlands;Dordrecht: Springer Science and Business Media LLC
Abstract:	摘要： RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in green and non-green seedlings (etiolated, cia5 - 2 , ispF and ispG albino mutants, lincomycin-, and norflurazon-treated). The editing efficiency of Arabidopsis plastid transcripts varies from site to site, and may be specifically regulated in different tissues. Steady state levels of plastid transcripts are low or undetectable in etiolated seedlings, but most editing sites are edited with efficiencies similar to those observed in green seedlings. By contrast, the editing of some sites is completely lost or significantly reduced in other non-green tissues; for instance, the editing of ndhB -149, ndhB -1255, and ndhD -2 is completely lost in roots and in lincomycin-treated seedlings. The editing of ndhD -2 is also completely lost in albino mutants and norflurazon-treated seedlings. However, matK -640 is completely edited, and accD -794, atpF -92, psbE -214, psbF -77, psbZ -50, and rps14 -50 are completely or highly edited in both green and non-green tissues. In addition, the expression of nucleus-encoded RNA polymerase dependent transcripts is specifically induced by lincomycin, and the splicing of ndhB transcripts is significantly reduced in the albino mutants and inhibitor-treated seedlings. Our results indicate that plastid gene expression, and the splicing and editing of plastid transcripts are specifically and differentially regulated in various types of non-green tissues. 其他題名： Plant Mol Biol 出版者： Dordrecht: Springer Science and Business Media LLC 出版日期： 2013-07-01 出處： Plant Molecular Biology, 2013-07, Vol.82 (4), p.375-392 版權： Springer Science+Business Media Dordrecht 2013 識別號： ISSN: 0167-4412 識別號： ISSN: 1573-5028 識別號： EISSN: 1573-5028 識別號： DOI: 10.1007/s11103-013-0069-5 識別號： PMID: 23645360
Appears in Collections:	[Department of Life Science] journal & Dissertation

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