Double homeobox gene, Duxbl, promotes myoblast proliferation and abolishes myoblast differentiation by blocking MyoD transactivation

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Title:	Double homeobox gene, Duxbl, promotes myoblast proliferation and abolishes myoblast differentiation by blocking MyoD transactivation
Authors:	陳盛良;Wu, Shey-Lin;Li, Guo-Zili;Chou, Chin-Yi;Tsai, Ming-Shiun;Chen, Yi-Pei;Li, Chung-Jung;Liou, Gan-Guang;Chang, Wen-Wei;Chen, Shen-Liang;Wang, Sue-Hong
Contributors:	生醫理工學院生命科學系
Keywords:	adults;Aging - metabolism;Animals;Biomedical and Life Sciences;Biomedicine;Cell Cycle Proteins - metabolism;Cell Differentiation - genetics;Cell growth;Cell Proliferation;Developmental biology;embryogenesis;Embryonic development;Fluorescent Antibody Technique;Gene expression;gene overexpression;Genes;Homeodomain Proteins - genetics;Homeodomain Proteins - metabolism;homeotic genes;Human Genetics;Humans;Luciferase;Mice;Molecular Medicine;Muscle Development;muscles;muscular dystrophy;Muscular system;myoblasts;Myoblasts - cytology;Myoblasts - metabolism;myocytes;MyoD Protein - genetics;MyoD Protein - metabolism;Myogenin - metabolism;organogenesis;Protein expression;proteins;Proteomics;Regeneration;Regular Article;RNA, Messenger - genetics;RNA, Messenger - metabolism;Satellite Cells, Skeletal Muscle - metabolism;Signal transduction;transcription factors;Transcription Factors - genetics;Transcription Factors - metabolism;transcriptional activation;Transcriptional Activation - genetics
Date:	2014-01-01
Issue Date:	2026-04-23 11:12:24 (UTC+8)
Publisher:	Springer Verlag;Berlin/Heidelberg: Springer-Verlag
Abstract:	摘要： Homeobox genes encode transcription factors that regulate embryonic development programs including organogenesis, axis formation and limb development. Previously, we identified and cloned a mouse double homeobox gene, Duxbl, whose homeodomain exhibits the highest identity (67 %) to human DUX4, a candidate gene of facioscapulohumeral muscular dystrophy (FSHD). Duxbl proteins have been shown to be expressed in elongated myocytes and myotubes of trunk and limb muscles during embryogenesis. In this study, we found that Duxbl maintained low expression levels in various adult muscles. Duxbl proteins were induced to express in activated satellite cells and colocalized with MyoG, a myogenic differentiating marker. Furthermore, Duxbl proteins were not detected in quiescent satellite cells but detected in regenerated myocytes and colocalized with MyoD and MyoG following cardiotoxin-induced muscle injury. Ectopic Duxbl overexpressions in C2C12 myoblast cells promoted cell proliferation through mainly enhancing cyclin D1 and hyper-phosphorylated retinoblastoma protein but reducing p21 expression. However, Duxbl overexpression in C2C12 cells inhibited myogenic differentiation by decreasing MyoD downstream gene expressions, including M-cadherin, MyoG, p21 and cyclin D3 but not MyoD itself. Duxbl overexpressions also promoted cell proliferation but blocked MyoD-induced myogenic conversion in multipotent mesenchymal C3H10T1/2 cells. In addition, results of a luciferase reporter assay suggest that Duxbl negatively regulated MyoG promoter activity through the proximal two E boxes. In conclusion, these results indicate that Duxbl may play a crucial role in myogenesis and postnatal muscle regeneration by activating and proliferating satellite and myoblast cells. 其他題名： Cell Tissue Res 出版者： Berlin/Heidelberg: Springer-Verlag 出版日期： 2014-11-01 出處： Cell and tissue research, 2014-11, Vol.358 (2), p.551-566 版權： Springer-Verlag Berlin Heidelberg 2014 版權： COPYRIGHT 2014 Springer 識別號： ISSN: 0302-766X 識別號： ISSN: 1432-0878 識別號： EISSN: 1432-0878 識別號： DOI: 10.1007/s00441-014-1974-x 識別號： PMID: 25130140
Appears in Collections:	[Department of Life Science] journal & Dissertation

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