Dysfunctional chloroplasts up-regulate the expression of mitochondrial genes in Arabidopsis seedlings

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Title:	Dysfunctional chloroplasts up-regulate the expression of mitochondrial genes in Arabidopsis seedlings
Authors:	謝明勳;Liao, Jo-Chien;Hsieh, Wei-Yu;Tseng, Ching-Chih;Hsieh, Ming-Hsiun
Contributors:	生醫理工學院生命科學系
Keywords:	Arabidopsis;Arabidopsis - drug effects;Arabidopsis - genetics;Arabidopsis - ultrastructure;Arabidopsis thaliana;Base Sequence;Biochemistry;Biomedical and Life Sciences;Chloroplasts;Chloroplasts - drug effects;Chloroplasts - metabolism;Chloroplasts - ultrastructure;electron transport chain;energy;Gene expression;Gene Expression Regulation, Plant - drug effects;genes;Genes, Mitochondrial;Genetic research;Genomes;Genomics;homeostasis;Introns - genetics;Life Sciences;lincomycin;Lincomycin - pharmacology;messenger RNA;mitochondria;Mitochondria - drug effects;Mitochondria - metabolism;Mitochondria - ultrastructure;Mitochondrial DNA;Molecular Sequence Data;norflurazon;nuclear genome;Photosynthesis;Photosynthesis - drug effects;Plant biochemistry;Plant Genetics and Genomics;Plant mitochondria;Plant Physiology;Plant Sciences;Pyridazines - pharmacology;Real-Time Polymerase Chain Reaction;Regular Paper;Ribonucleic acid;RNA;RNA editing;RNA Editing - genetics;RNA Splicing - drug effects;RNA Splicing - genetics;RNA, Messenger - genetics;RNA, Messenger - metabolism;seedlings;Seedlings - drug effects;Seedlings - genetics;Seedlings - ultrastructure;Up-Regulation - drug effects;Up-Regulation - genetics
Date:	2016-02-01
Issue Date:	2026-04-23 11:12:26 (UTC+8)
Publisher:	Springer Netherlands;Dordrecht: Springer Netherlands
Abstract:	摘要： Chloroplasts and mitochondria play important roles in maintaining metabolic and energy homeostasis in the plant cell. The interactions between these two organelles, especially photosynthesis and respiration, have been intensively studied. Still, little is known about the regulation of mitochondrial gene expression by chloroplasts and vice versa. The gene expression machineries in chloroplasts and mitochondria rely heavily on the nuclear genome. Thus, the interactions between nucleus and these organelles, including anterograde and retrograde regulation, have been actively investigated in the last two decades. Norflurazon (NF) and lincomycin (Lin) are two commonly used inhibitors to study chloroplast-to-nucleus retrograde signaling in plants. We used NF and Lin to block the development and functions of chloroplasts and examined their effects on mitochondrial gene expression, RNA editing and splicing. The editing of most mitochondrial transcripts was not affected, but the editing extents of nad4-107, nad6-103, and ccmFc-1172 decreased slightly in NF- and Lin-treated seedlings. While the splicing of mitochondrial transcripts was not significantly affected, steady-state mRNA levels of several mitochondrial genes increased significantly in NF- and Lin-treated seedlings. Moreover, Lin seemed to have more profound effects than NF on the expression of mitochondrial genes, indicating that signals derived from these two inhibitors might be distinct. NF and Lin also significantly induced the expression of nuclear genes encoding subunits of mitochondrial electron transport chain complexes. Thus, dysfunctional chloroplasts may coordinately up-regulate the expression of nuclear and mitochondrial genes encoding subunits of respiratory complexes. 其他題名： Photosynth Res 出版者： Dordrecht: Springer Netherlands 出版日期： 2016-02-01 出處： Photosynthesis research, 2016-02, Vol.127 (2), p.151-159 版權： Springer Science+Business Media Dordrecht 2015 版權： COPYRIGHT 2016 Springer 版權： Springer Science+Business Media Dordrecht 2016 識別號： ISSN: 0166-8595 識別號： EISSN: 1573-5079 識別號： DOI: 10.1007/s11120-015-0161-6 識別號： PMID: 26008795
Appears in Collections:	[Department of Life Science] journal & Dissertation

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