Efficient secretion of recombinant proteins from rice suspension-cultured cells modulated by the choice of signal peptide

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題名:	Efficient secretion of recombinant proteins from rice suspension-cultured cells modulated by the choice of signal peptide
作者:	陸重安;Huang, Li-Fen;Tan, Chia-Chun;Yeh, Ju-Fang;Liu, Hsin-Yi;Liu, Yu-Kuo;Ho, Shin-Lon;Lu, Chung-An
貢獻者:	生醫理工學院生命科學系
關鍵詞:	Amino acids;Animals;Bioengineering;Cell culture;Cell Culture Techniques;Cell Line, Tumor;Colony-stimulating factor;Efficiency;Fluorescence;Gene expression;Genetic engineering;Granulocyte-macrophage colony-stimulating factor;Granulocyte-Macrophage Colony-Stimulating Factor - genetics;Granulocyte-Macrophage Colony-Stimulating Factor - secretion;Granulocytes;Green fluorescent protein;Leukemia;Life sciences;Macrophages;Mice;Myeloblastic leukemia;N-Terminus;Oryza;Oryza - cytology;Oryza - genetics;Oryza - metabolism;Oryza sativa;Peptides;Plant Cells - secretion;Plastids;Protein expression;Protein Sorting Signals - physiology;Proteins;Recombinant proteins;Recombinant Proteins - genetics;Recombinant Proteins - secretion;Rice;Secretion;Signal peptides;Transgenic
日期:	2015-10-16
上傳時間:	2026-04-23 11:12:29 (UTC+8)
出版者:	Public Library of Science;United States: Public Library of Science
摘要:	摘要： Plant-based expression systems have emerged as a competitive platform in the large-scale production of recombinant proteins. By adding a signal peptide, αAmy3sp, the desired recombinant proteins can be secreted outside transgenic rice cells, making them easy to harvest. In this work, to improve the secretion efficiency of recombinant proteins in rice expression systems, various signal peptides including αAmy3sp, CIN1sp, and 33KDsp have been fused to the N-terminus of green fluorescent protein (GFP) and introduced into rice cells to explore the efficiency of secretion of foreign proteins. 33KDsp had better efficiency than αAmy3sp and CIN1sp for the secretion of GFP from calli and suspension-cultured cells. 33KDsp was further applied for the secretion of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) from transgenic rice suspension-cultured cells; approximately 76%-92% of total rice-derived mGM-CSF (rmGM-CSF) was detected in the culture medium. The rmGM-CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell line, NSF-60. The extracellular yield of rmGM-CSF reached 31.7 mg/L. Our study indicates that 33KDsp is better at promoting the secretion of recombinant proteins in rice suspension-cultured cell systems than the commonly used αAmy3sp. 其他題名： PLoS One 出版者： United States: Public Library of Science 出版日期： 2015-10-16 出處： PloS one, 2015-10, Vol.10 (10), p.e0140812-e0140812 資源來源： Agricultural & Environmental Science Collection 版權： COPYRIGHT 2015 Public Library of Science 版權： 2015 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2015 Huang et al 2015 Huang et al 識別號： ISSN: 1932-6203 識別號： EISSN: 1932-6203 識別號： DOI: 10.1371/journal.pone.0140812 識別號： PMID: 26473722
顯示於類別:	[生命科學系] 期刊論文

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