Ellagic acid protects human keratinocyte (HaCaT) cells against UVA-induced oxidative stress and apoptosis through the upregulation of the HO-1 and Nrf-2 antioxidant genes

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Title:	Ellagic acid protects human keratinocyte (HaCaT) cells against UVA-induced oxidative stress and apoptosis through the upregulation of the HO-1 and Nrf-2 antioxidant genes
Authors:	陳師慶;Hseu, You-Cheng;Chou, Chih-Wei;Senthil Kumar, K.J.;Fu, Ke-Ting;Wang, Hui-Min;Hsu, Li-Sung;Kuo, Yueh-Hsiung;Wu, Chi-Rei;Chen, Ssu-Ching;Yang, Hsin-Ling
Contributors:	生醫理工學院生命科學系
Keywords:	acid treatment;antioxidant activity;antioxidants;Apoptosis;Apoptosis - drug effects;Apoptosis - radiation effects;Biological and medical sciences;caspase-3;Cell Line, Transformed;cell viability;Chemical mutagenesis;Comet Assay;DNA Damage;DNA fragmentation;Ellagic acid;Ellagic Acid - pharmacology;genes;Heme Oxygenase (Decyclizing) - genetics;Heme oxygenase 1;Humans;irradiation;keratinocytes;Keratinocytes - drug effects;Keratinocytes - metabolism;Keratinocytes - radiation effects;Lipid Peroxidation;Medical sciences;mitochondria;NF-E2-Related Factor 2 - genetics;NF-E2-related factor-2;oxidative stress;Oxidative Stress - drug effects;pathogenesis;pretreatment;protective effect;Reactive oxygen species;Reactive Oxygen Species - metabolism;risk factors;RNA, Small Interfering;superoxide dismutase;Toxicology;transcriptional activation;Ultraviolet A;ultraviolet radiation;Ultraviolet Rays;Up-Regulation - drug effects
Date:	2012-05-01
Issue Date:	2026-04-23 11:12:30 (UTC+8)
Publisher:	Elsevier Ltd.;Oxford: Elsevier Ltd
Abstract:	摘要： ► Ellagic acid treatment suppressed UVA-induced ROS generation and MDA formation. ► Ellagic acid treatment inhibited UVA-induced apoptosis in HaCaT cells. ► The antioxidant potential of ellagic acid was correlated with HO-1 expression. ► Ellagic acid-induced HO-1 expression was mediated by up-regulation of Nrf2. ► Nrf2 knockdown diminishes the protective effect of ellagic acid. UV radiation from the sun is a potent environmental risk factor in the pathogenesis of skin damage. Much of the skin damage caused by ultraviolet A (UVA) irradiation from the sun is associated with oxidative stress. The aim of this study was to investigate the protective role of ellagic acid (25–75μM), a natural antioxidant, against UVA (5–20J/cm2)-induced oxidative stress and apoptosis in human keratinocyte (HaCaT) cells and to reveal the possible mechanisms underlying this protective efficacy. Ellagic acid pre-treatment markedly increased HaCaT cell viability and suppressed UVA-induced ROS generation and MDA formation. Moreover, ellagic acid pre-treatment prevented UVA-induced DNA damage as evaluated by the comet assay. Ellagic acid treatment also significantly inhibited the UVA-induced apoptosis of HaCaT cells, as measured by a reduction of DNA fragmentation, mitochondria dysfunction, ER stress, caspase-3 activation, and Bcl-2/Bax deregulation. Notably, the antioxidant potential of ellagic acid was directly correlated with the increased expression of HO-1 and SOD, which was followed by the downregulation of Keap1 and the augmented nuclear translocation and transcriptional activation of Nrf2 with or without UVA irradiation. Nrf2 knockdown diminished the protective effects of ellagic acid. Therefore, ellagic acid may be useful for the treatment of UVA-induced skin damage. 其他題名： Food Chem Toxicol 出版者： Oxford: Elsevier Ltd 出版日期： 2012-05-01 出處： Food and Chemical Toxicology, 2012-05, Vol.50 (5), p.1245-1255 版權： 2012 Elsevier Ltd 版權： 2015 INIST-CNRS 版權： Copyright © 2012 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0278-6915 識別號： ISSN: 1873-6351 識別號： EISSN: 1873-6351 識別號： DOI: 10.1016/j.fct.2012.02.020 識別號： PMID: 22386815 識別號： CODEN: FCTOD7
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