Everolimus sensitizes Ras-transformed cells to radiation in vitro through the autophagy pathway

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題名:	Everolimus sensitizes Ras-transformed cells to radiation in vitro through the autophagy pathway
作者:	許斐婷;Su, Yu-Chieh;Yu, Chih-Chia;Hsu, Fei-Ting;Fu, Shu-Ling;Hwang, Jeng-Jong;Hung, Ling-Chien;Lee, Moon-Sing;Chiou, Wen- Yen;Lin, Hon-Yi;Hung, Shih-Kai
貢獻者:	生醫理工學院生命科學系
關鍵詞:	Autophagy (Cytology);Health aspects;Radiotherapy
日期:	2014-11-01
上傳時間:	2026-04-23 11:12:38 (UTC+8)
出版者:	Spandidos Publications
摘要:	摘要： Modern radiation therapy strives to minimize injury to organs while increasing the anticancer effects. The present study aimed to investigate the radiosensitizing effects of everolimus and to examine the molecular mechanisms responsible for everolimus-mediated radiosensitization. Radiation in combination with everolimus (30 nM) sensitized Ras-transformed cells to radiation in vitro. Radiation induced apoptotic markers (sub-G1 cell accumulation, membrane inversion and DNA fragmentation) and treatment with everolimus did not promote radiation-induced apoptosis. However, LC3-II expression increased following combination treatment with everolimus and radiation, and the radiosensitizing effects of everolimus were reversed following transfection with small interfering RNA (siRNA) targeting Beclin 1. In addition, the protein levels of activated S6 kinase 1 (S6K1) were significantly reduced following treatment with everolimus, and the phosphorylation of factor 4E binding protein 1 (4EBP1) was suppressed following combination treatment. Taken together, our data demonstrate that everolimus sensitizes Ras-transformed cells to radiation in vitro. Everolimus-mediated radiosensitization is associated with the autophagy pathway. Thus, everolimus is a novel radio-sensitizing agent with potential for use in cancer radiotherapy. 出版者： Spandidos Publications 出版日期： 2014-11-01 出處： International journal of molecular medicine, 2014-11, Vol.34 (5), p.1417 版權： COPYRIGHT 2014 Spandidos Publications 識別號： ISSN: 1107-3756 識別號： DOI: 10.3892/ijmm.2014.1927
顯示於類別:	[生命科學系] 期刊論文

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