Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening

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Title:	Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening
Authors:	葉靖輝;Hsu, Kuo-Hsuan;Liu, Chia-Chin;Wu, Shaw-Jye;Kuo, Ying-Yu;Lu, Chung-An;Wu, Ching-Rong;Lian, Pei-Jyun;Hong, Chwan-Yang;Ke, Yi-Ting;Huang, Juin-Hua;Yeh, Ching-Hui
Contributors:	生醫理工學院生命科學系
Keywords:	Abiotic stress;abscisic acid;Abscisic Acid - pharmacology;Amino Acid Sequence;Arabidopsis;Arabidopsis - genetics;Biochemistry;Biomedical and Life Sciences;calcium;Cloning, Molecular;Cooling;drug effects;Evaporation;Evaporation rate;Gene expression;Gene Expression Profiling;gene expression regulation;Gene Expression Regulation, Plant;Gene Expression Regulation, Plant - drug effects;gene overexpression;genes;genetics;Heat-Shock Response;Heat-Shock Response - genetics;High temperature;Leaves;Life Sciences;metabolism;microarray technology;Molecular Sequence Data;mutants;mutation;Oryza;Oryza - drug effects;Oryza - genetics;Oryza - metabolism;Oryza - ultrastructure;Oryza sativa;pharmacology;phenotype;physiology;Plant biology;Plant Pathology;Plant Proteins;Plant Proteins - genetics;Plant Proteins - metabolism;Plant Proteins - physiology;Plant Sciences;Plant Stomata;Plant Stomata - drug effects;Plant Stomata - physiology;potassium;Proteins;Rice;Sequence Alignment;Stomata;stomatal movement;Temperature;ultrastructure;Water analysis;Water loss;Zinc Fingers
Date:	2014-01-01
Issue Date:	2026-04-23 11:12:43 (UTC+8)
Publisher:	Springer Netherlands;Dordrecht: Springer-Verlag
Abstract:	摘要： By oligo microarray expression profiling, we identified a rice RING zinc-finger protein (RZFP), OsRZFP34, whose gene expression increased with high temperature or abscisic acid (ABA) treatment. As compared with the wild type, rice and Arabidopsis with OsRZFP34 overexpression showed increased relative stomata opening even with ABA treatment. Furthermore, loss-of-function mutation of OsRZFP34 and AtRZFP34 (At5g22920), an OsRZFP34 homolog in Arabidopsis, decreased relative stomata aperture under nonstress control conditions. Expressing OsRZFP34 in atrzfp34 reverted the mutant phenotype to normal, which indicates a conserved molecular function between OsRZFP34 and AtRZFP34. Analysis of water loss and leaf temperature under stress conditions revealed a higher evaporation rate and cooling effect in OsRZFP34-overexpressing Arabidopsis and rice than the wild type, atrzfp34 and osrzfp34. Thus, stomata opening, enhanced leaf cooling, and ABA insensitivity was conserved with OsRZFP34 expression. Transcription profiling of transgenic rice overexpressing OsRZFP34 revealed many genes involved in OsRZFP34-mediated stomatal movement. Several genes upregulated or downregulated in OsRZFP34-overexpressing plants were previously implicated in Ca²⁺ sensing, K⁺ regulator, and ABA response. We suggest that OsRZFP34 may modulate these genes to control stomata opening. 其他題名： Plant Mol Biol 出版者： Dordrecht: Springer-Verlag 出版日期： 2014-09-01 出處： Plant molecular biology, 2014-09, Vol.86 (1-2), p.125-137 版權： Springer Science+Business Media Dordrecht 2014 識別號： ISSN: 0167-4412 識別號： ISSN: 1573-5028 識別號： EISSN: 1573-5028 識別號： DOI: 10.1007/s11103-014-0217-6 識別號： PMID: 25002225
Appears in Collections:	[Department of Life Science] journal & Dissertation

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