Functional divergence of the miRNA transcriptome at the onset of drosophila metamorphosis

NCUIR > college of Health Sciences and Technology > Department of Life Science > journal & Dissertation > Item 987654321/102554

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/102554

Title:	Functional divergence of the miRNA transcriptome at the onset of drosophila metamorphosis
Authors:	葉淑丹;Yeh, Shu-Dan;von Grotthuss, Marcin;Gandasetiawan, Kania A.;Jayasekera, Suvini;Xia, Xiao-Qin;Chan, Carolus;Jayaswal, Vivek;Ranz, José M.
Contributors:	生醫理工學院生命科學系
Keywords:	Animals;Biological evolution;Conserved Sequence;Developmental stages;Drosophila;Drosophila melanogaster;Drosophila melanogaster - classification;Drosophila melanogaster - genetics;Drosophila melanogaster - growth & development;Ecdysone;Evolution, Molecular;Female;Fruit flies;Gene expression;Gene Expression Profiling;Gene Expression Regulation, Developmental;Genetic Variation;Insects;Male;Metamorphosis;Metamorphosis, Biological;MicroRNAs;MicroRNAs - genetics;miRNA;Molecular biology;Molecular Sequence Data;Molecules;Ontogeny;Phylogeny;Polymorphism;Sex Characteristics;Transcriptomes;Variation
Date:	2014-10-01
Issue Date:	2026-04-23 11:12:47 (UTC+8)
Publisher:	Oxford University Press;United States: Oxford University Press
Abstract:	摘要： MicroRNAs (miRNAs) are endogenous RNA molecules that regulate gene expression posttranscriptionally. To date, the emergence of miRNAs and their patterns of sequence evolution have been analyzed in great detail. However, the extent to which miRNA expression levels have evolved over time, the role different evolutionary forces play in shaping these changes, and whether this variation in miRNA expression can reveal the interplay between miRNAs and mRNAs remain poorly understood. This is especially true for miRNA expressed during key developmental transitions. Here, we assayed miRNA expression levels immediately before (≥18BPF [18 h before puparium formation]) and after (PF) the increase in the hormone ecdysone responsible for triggering metamorphosis. We did so in four strains of Drosophila melanogaster and two closely related species. In contrast to their sequence conservation, approximately 25% of miRNAs analyzed showed significant within-species variation in male expression levels at ≥18BPF and/or PF. Additionally, approximately 33% showed modifications in their pattern of expression bias between developmental timepoints. A separate analysis of the ≥18BPF and PF stages revealed that changes in miRNA abundance accumulate linearly over evolutionary time at PF but not at ≥18BPF. Importantly, ≥18BPF-enriched miRNAs showed the greatest variation in expression levels both within and between species, so are the less likely to evolve under stabilizing selection. Functional attributes, such as expression ubiquity, appeared more tightly associated with lower levels of miRNA expression polymorphism at PF than at ≥18BPF. Furthermore, ≥18BPF- and PF-enriched miRNAs showed opposite patterns of covariation in expression with mRNAs, which denoted the type of regulatory relationship between miRNAs and mRNAs. Collectively, our results show contrasting patterns of functional divergence associated with miRNA expression levels during Drosophila ontogeny. 其他題名： Mol Biol Evol 出版者： United States: Oxford University Press 出版日期： 2014-10-01 出處： Molecular biology and evolution, 2014-10, Vol.31 (10), p.2557-2572 版權： The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com 2014 版權： The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. 版權： Copyright Oxford Publishing Limited(England) Oct 2014 識別號： ISSN: 0737-4038 識別號： ISSN: 1537-1719 識別號： EISSN: 1537-1719 識別號： DOI: 10.1093/molbev/msu195 識別號： PMID: 24951729
Appears in Collections:	[Department of Life Science] journal & Dissertation

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	22	View/Open

社群 sharing

Loading...