Functional substitution of a eukaryotic glycyl-trna synthetase with an evolutionarily unrelated bacterial cognate enzyme

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題名:	Functional substitution of a eukaryotic glycyl-trna synthetase with an evolutionarily unrelated bacterial cognate enzyme
作者:	王健家;Chien, Chin-I;Chen, Yu-Wei;Wu, Yi-Hua;Chang, Chih-Yao;Wang, Tzu-Ling;Wang, Chien-Chia
貢獻者:	生醫理工學院生命科學系
關鍵詞:	Amino acids;Aminoacylation;Animals;Arabidopsis thaliana;Bacteria;Bacteria - enzymology;Baking yeast;Base Sequence;Biology and Life Sciences;Chloroplasts;Cloning, Molecular;Compartments;Cytoplasm;Defects;E coli;Enzymes;Escherichia coli;Eukaryota - enzymology;Evolution, Molecular;Gene Knockout Techniques;Genes;Genetic aspects;Glycine-tRNA ligase;Glycine-tRNA Ligase - chemistry;Glycine-tRNA Ligase - deficiency;Glycine-tRNA Ligase - genetics;Glycine-tRNA Ligase - metabolism;Humans;Isoacceptors;Life sciences;Mitochondria;Mitochondria - genetics;Mitochondria - metabolism;Molecular evolution;Physiological aspects;Plant mitochondria;Protein Multimerization;Protein Structure, Quaternary;Protein Transport;Proteins;RNA polymerases;Saccharomyces cerevisiae;Transfer RNA;tRNA;Yeast
日期:	2014-04-17
上傳時間:	2026-04-23 11:12:51 (UTC+8)
出版者:	Public Library of Science;United States: Public Library of Science
摘要:	摘要： Two oligomeric types of glycyl-tRNA synthetase (GlyRS) are found in nature: a α2 type and a α2β2 type. The former has been identified in all three kingdoms of life and often pairs with tRNAGly that carries an A73 discriminator base, while the latter is found only in bacteria and chloroplasts and is almost always coupled with tRNAGly that contains U73. In the yeast Saccharomyces cerevisiae, a single GlyRS gene, GRS1, provides both the cytoplasmic and mitochondrial functions, and tRNAGly isoacceptors in both compartments possess A73. We showed herein that Homo sapiens and Arabidopsis thaliana cytoplasmic GlyRSs (both α2-type enzymes) can rescue both the cytoplasmic and mitochondrial defects of a yeast grs1- strain, while Escherichia coli GlyRS (a α2β2-type enzyme) and A. thaliana organellar GlyRS (a (αβ)2-type enzyme) failed to rescue either defect of the yeast mull allele. However, a head-to-tail αβ fusion of E. coli GlyRS effectively supported the mitochondrial function. Our study suggests that a α2-type eukaryotic GlyRS may be functionally substituted with a α2β2-type bacterial cognate enzyme despite their remote evolutionary relationships. 其他題名： PLoS One 出版者： United States: Public Library of Science 出版日期： 2014-04-01 出處： PloS one, 2014-04, Vol.9 (4), p.e94659 資源來源： Agricultural & Environmental Science Collection 版權： COPYRIGHT 2014 Public Library of Science 版權： 2014 Chien et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2014 Chien et al 2014 Chien et al 識別號： ISSN: 1932-6203 識別號： EISSN: 1932-6203 識別號： DOI: 10.1371/journal.pone.0094659 識別號： PMID: 24743154
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