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| 題名: | High-fat diet—induced retinal dysfunction |
| 作者: | 黃佳瑜;Chang, Richard Cheng-An;Shi, Liheng;Huang, Cathy Chia-Yu;Kim, Andy Jeesu;Ko, Michael L.;Zhou, Beiyan;Ko, Gladys Y.-P. |
| 貢獻者: | 生醫理工學院生命科學系 |
| 關鍵詞: | Animals;Blotting, Western;Calcium Channels, L-Type - metabolism;Diet, High-Fat - adverse effects;Electroretinography;Glucose Transporter Type 4 - metabolism;Humans;Immunohistochemistry;Male;Mice;Mice, Inbred C57BL;Plasma Membrane Calcium-Transporting ATPases - metabolism;Proto-Oncogene Proteins c-akt - metabolism;Retina;Retinal Diseases - diagnosis;Retinal Diseases - etiology;Retinal Diseases - metabolism |
| 日期: | 2015-01-01 |
| 上傳時間: | 2026-04-23 11:13:01 (UTC+8) |
| 出版者: | United States: The Association for Research in Vision and Ophthalmology |
| 摘要: | 摘要： The purpose of this study was to investigate the impact of obesity-induced prediabetes/early diabetes on the retina to provide new evidence on the pathogenesis of type 2 diabetes-associated diabetic retinopathy (DR). A high-fat diet (HFD)-induced obesity mouse model (male C57BL/6J) was used in this study. At the end of the 12-week HFD feeding regimen, mice were evaluated for glucose and insulin tolerance, and retinal light responses were recorded by electroretinogram (ERG). Western immunoblot and immunohistochemical staining were used to determine changes in elements regulating calcium homeostasis between HFD and control retinas, as well as unstained human retinal sections from DR patients and age-appropriate controls. Compared to the control, the scotopic and photopic ERGs from HFD mice were decreased. There were significant decreases in molecules related to cell signaling, calcium homeostasis, and glucose metabolism from HFD retinas, including phosphorylated protein kinase B (pAKT), glucose transporter 4, L-type voltage-gated calcium channel (L-VGCC), and plasma membrane calcium ATPase (PMCA). Similar changes for pAKT, PMCA, and L-VGCC were also observed in human retinal sections from DR patients. Obesity-induced hyperglycemic and prediabetic/early diabetic conditions caused detrimental impacts on retinal light sensitivities and health. The decrease of the ERG components in early diabetes reflects the decreased neuronal activity of retinal light responses, which may be caused by a decrease in neuronal calcium signaling. Since PI3K-AKT is important in regulating calcium homeostasis and neural survival, maintaining proper PI3K-AKT signaling in early diabetes or at the prediabetic stage might be a new strategy for DR prevention.
其他題名： Invest Ophthalmol Vis Sci
出版者： United States: The Association for Research in Vision and Ophthalmology
出版日期： 2015-04-01
出處： Investigative ophthalmology & visual science, 2015-04, Vol.56 (4), p.2367-2380
版權： Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc. 2015
識別號： ISSN: 1552-5783
識別號： ISSN: 0146-0404
識別號： EISSN: 1552-5783
識別號： DOI: 10.1167/iovs.14-16143
識別號： PMID: 25788653 |
| 顯示於類別: | [生命科學系] 期刊論文
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