Identification and characterization of a novel chloroplast/mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice

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Title:	Identification and characterization of a novel chloroplast/mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice
Authors:	葉靖輝;Wu, Tsung-Meng;Lin, Wan-Rong;Kao, Yun-Ting;Hsu, Yi-Ting;Yeh, Ching-Hui;Hong, Chwan-Yang;Kao, Ching Huei
Contributors:	生醫理工學院生命科學系
Keywords:	Amino Acid Sequence;Antioxidants;Biochemistry;Biomedical and Life Sciences;biotic stress;Chloroplasts;Chloroplasts - enzymology;Cloning;complementary DNA;Conserved Sequence;Dehydration;drug effects;E coli;enzymology;Escherichia coli;Escherichia coli - genetics;Escherichia coli - metabolism;Gene Expression;Gene Expression Regulation, Plant;Gene Expression Regulation, Plant - drug effects;genetic transformation;genetics;Glutathione Reductase;Glutathione Reductase - drug effects;Glutathione Reductase - genetics;Glutathione Reductase - metabolism;glutathione-disulfide reductase;heat;Isoenzymes;Life Sciences;metabolism;methyl jasmonate;Mitochondria;Mitochondria - enzymology;Molecular Sequence Data;Oryza;Oryza - drug effects;Oryza - enzymology;Oryza - genetics;Oryza - ultrastructure;pharmacology;Phylogeny;Plant biology;Plant Growth Regulators;Plant Growth Regulators - pharmacology;Plant Pathology;Plant Proteins;Plant Proteins - drug effects;Plant Proteins - genetics;Plant Proteins - metabolism;Plant Roots;Plant Roots - drug effects;Plant Roots - enzymology;Plant Roots - genetics;Plant Roots - ultrastructure;Plant Sciences;Plant Shoots;Plant Shoots - drug effects;Plant Shoots - enzymology;Plant Shoots - genetics;Plant Shoots - ultrastructure;Plants, Genetically Modified;Poaceae;proteins;Recombinant Fusion Proteins;Rice;salicylic acid;Salicylic Acid - pharmacology;Salinity;Salt;salt stress;Salts;Seedlings;Seedlings - drug effects;Seedlings - enzymology;Seedlings - genetics;Seedlings - ultrastructure;Sequence Alignment;Sodium Chloride;Sodium Chloride - pharmacology;Stress analysis;stress response;stress tolerance;Stress, Physiological;Stress, Physiological - drug effects;ultrastructure
Date:	2013-06-20
Issue Date:	2026-04-23 11:13:02 (UTC+8)
Publisher:	Springer Netherlands;Dordrecht: Springer Science and Business Media LLC
Abstract:	摘要： Glutathione reductases (GRs) are important components of the antioxidant machinery that plants use to respond against abiotic stresses. In rice, one cytosolic and two chloroplastic GR isoforms have been identified. In this work, we describe the cloning and characterization of the full-length cDNA encoding OsGR3 , a chloroplast-localized GR that up to now was considered as a non-functional enzyme because of assumed lack of N-terminal conserved domains. The expression of OsGR3 in E. coli validated that it can be translated as a protein with GR activity. OsGR3 shows 76 and 53 % identity with OsGR1 (chloroplastic) and OsGR2 (cytosolic), respectively. Phylogenetic analysis revealed 2 chloroplastic GRs in Poaceae species, including rice, sorghum and brachypodium, but only one chloroplastic GR in dicots. A plastid transit peptide is located at the N terminus of OsGR3, and genetic transformation of rice with a GR3-GFP fusion construct further confirmed its localization in chloroplasts. Furthermore, OsGR1 and OsGR3 are also targeted to mitochondria, which suggest a combined antioxidant mechanism in both chloroplasts and mitochondria. However, both isoforms showed a distinct response to salinity: the expression of OsGR3 but not OsGR1 was induced by salt stress. In addition, the transcript level of OsGR3 was greatly increased with salicylic acid treatment but was not significantly affected by methyl jasmonate, dehydration or heat shock stress. Our results provide new clues about the possible roles of functional OsGR3 in salt stress and biotic stress tolerance. 其他題名： Plant Mol Biol 出版者： Dordrecht: Springer Science and Business Media LLC 出版日期： 2013-11-01 出處： Plant Molecular Biology, 2013-11, Vol.83 (4), p.379-390 資源來源： PubMed 版權： Springer Science+Business Media Dordrecht 2013 識別號： ISSN: 0167-4412 識別號： ISSN: 1573-5028 識別號： EISSN: 1573-5028 識別號： DOI: 10.1007/s11103-013-0095-3 識別號： PMID: 23783412
Appears in Collections:	[Department of Life Science] journal & Dissertation

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