Induction of macrophage cell-cycle arrest and apoptosis by humic acid

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Title:	Induction of macrophage cell-cycle arrest and apoptosis by humic acid
Authors:	陳師慶;Yang, Hsin-Ling;Huang, Pei-Jane;Chen, Ssu-Ching;Cho, Hsin-Ju;Kumar, K. J. Senthil;Lu, Fung-Jou;Chen, Chih-Sheng;Chang, Chia-Ting;Hseu, You-Cheng
Contributors:	生醫理工學院生命科學系
Keywords:	Animals;apoptosis;Apoptosis - drug effects;Bioassays;blackfoot disease;Caspase 3 - metabolism;Caspase 9 - metabolism;cdc25 Phosphatases - metabolism;Cell Cycle Checkpoints - drug effects;Cell Line - drug effects;Cell Survival - drug effects;Cyclin A - metabolism;Cyclin B1 - metabolism;Cytochromes c - metabolism;Deoxyribonucleic acid;DNA;DNA damage;DNA Fragmentation - drug effects;Endoplasmic Reticulum Stress;G2 Phase Cell Cycle Checkpoints - drug effects;G2/M arrest;humic acid;Humic acids;Humic Substances - toxicity;Immune system;Macrophages - drug effects;Macrophages - pathology;Mice;Mitochondria - metabolism;Well water
Date:	2014-01-01
Issue Date:	2026-04-23 11:13:07 (UTC+8)
Publisher:	Wiley-Liss Inc.;United States: Blackwell Publishing Ltd
Abstract:	摘要： Humic acid (HA) in well water is associated with Blackfoot disease and various cancers. Previously, we reported that acute humic acid exposure (25–200 µg/mL for 24 hr) induces inflammation in RAW264.7 macrophages. In this study, we observed that prolonged (72 hr) HA exposure (25–200 µg/mL) induces cell‐cycle arrest and apoptosis in cultured RAW264.7 cells. We also observed that exposing macrophages to HA arrests cells in the G2/M phase of the cell cycle by reducing cyclin A/B1, Cdc2, and Cdc25C levels. Treating macrophages with HA triggers a sequence of events characteristic of apoptotic cell death including loss of cell viability, morphological changes, internucleosomal DNA fragmentation, sub‐G1 accumulation. Molecular markers of apoptosis associated with mitochondrial dysfunction were similarly observed, including cytochrome c release, caspase‐3 or caspase‐9 activation, and Bcl‐2/Bax dysregulation. In addition to the mitochondrial pathway, HA‐induced apoptosis may also be mediated through the death receptor and ER stress pathways, as evidence by induction of Fas, caspase‐8, caspase‐4, and caspase‐12 activity. HA also upregulates p53 expression and causes DNA damage as assessed by the comet assay. These findings yield new insight into the mechanisms by which HA exposure may trigger atherosclerosis through modulation of the macrophage‐mediated immune system. Environ. Mol. Mutagen. 55:741–750, 2014. © 2014 Wiley Periodicals, Inc. 其他題名： Environ. Mol. Mutagen 出版者： United States: Blackwell Publishing Ltd 出版日期： 2014-12 出處： Environmental and molecular mutagenesis, 2014-12, Vol.55 (9), p.741-750 資源來源： Wiley Online Library - AutoHoldings Journals 版權： 2014 Wiley Periodicals, Inc. 識別號： ISSN: 0893-6692 識別號： ISSN: 1098-2280 識別號： EISSN: 1098-2280 識別號： DOI: 10.1002/em.21897 識別號： PMID: 25179584
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