資料載入中.....
|
請使用永久網址來引用或連結此文件:
https://ir.lib.ncu.edu.tw/handle/987654321/102578
|
| 題名: | Inhibition of cell growth and induction of apoptosis by Antrodia camphorata in HER-2/neu-overexpressing breast cancer cells through the induction of ROS, depletion of HER-2/neu, and disruption of the PI3K/Akt signaling pathway |
| 作者: | 陳師慶;Hseu, You Cheng;Chen, Ssu-Ching;Lin, Kai-Yuan;Cho, Hsin-Ju;Juan, Ying-Chen;Kumar, K. J. Senthil;Way, Tzong-Der;Yang, Hsin-Ling;Lee, Chuan-Chen;Hsu, Li-Sung |
| 貢獻者: | 生醫理工學院生命科學系 |
| 關鍵詞: | 1-Phosphatidylinositol 3-kinase;Acetylcysteine;AKT protein;Antioxidants;Antitumor activity;Apoptosis;Bcl-2 protein;Breast cancer;Cancer therapies;Caspase;Caspase-3;Cell culture;Cell death;Cell growth;Colorectal cancer;Cyclin D1;Cyclin E;Cyclin-dependent kinase 4;Cytochrome c;Cytotoxicity;DNA fragmentation;ErbB-2 protein;Fermentation;Kinases;Mitochondrial DNA;Penicillin;Phosphorylation;Poly(ADP-ribose) polymerase;Reactive oxygen species;Signal transduction;Tumors |
| 日期: | 2012-07-16 |
| 上傳時間: | 2026-04-23 11:13:08 (UTC+8) |
| 出版者: | Cairo, Egypt: Hindawi Publishing Corporation |
| 摘要: | 摘要： Previously, we demonstrated that a submerged fermentation culture of Antrodia camphorata (AC) promotes cell-cycle arrest and apoptosis in human estrogen receptor-positive/negative breast cancer cells. However, whether AC is effective against HER-2/neu-overexpressing breast cancers has not been thoroughly elucidated. In the present study, we showed that AC exhibited a significant cytotoxic effect against HER-2/neu-overexpressing MDA-MB-453 and BT-474 cells. Immunoblot analysis demonstrated that HER-2/neu and their tyrosine phosphorylation were inhibited by AC in a dose-dependent manner. An increase in intracellular reactive oxygen species (ROS) was observed in AC-treated cells, whereas antioxidant N-acetylcysteine (NAC) significantly prevented AC induced HER-2/neu depletion and cell death, which directly indicates that AC-induced HER-2/neu depletion and cell death was mediated by ROS generation. Also, AC significantly downregulated the expression of cyclin D1, cyclin E, and CDK4 followed by the suppression of PI3K/Akt, and their downstream effectors GSK-3β and β-catenin. Notably, AC-treatment induced apoptotic cell death, which was associated with sub-G1 accumulation, DNA fragmentation, mitochondrial dysfunction, cytochrome c release, caspase-3/-9 activation, PARP degradation, and Bcl-2/Bax dysregulation. Assays for colony formation also confirmed the growth-inhibitory effects of AC. This is the first report confirming the anticancer activity of this potentially beneficial mushroom against human HER-2/neu-overexpressing breast cancers.
其他題名： Evid Based Complement Alternat Med
出版者： Cairo, Egypt: Hindawi Publishing Corporation
出版日期： 2012-01-01
出處： Evidence-based complementary and alternative medicine, 2012-01, Vol.2012 (2012), p.1-15
資源來源： ProQuest Open Access Content Collection
版權： Copyright © 2012 Chuan-Chen Lee et al.
版權： Copyright © 2012 Chuan-Chen Lee et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0
版權： Copyright © 2012 Chuan-Chen Lee et al. 2012
識別號： ISSN: 1741-427X
識別號： ISSN: 1741-4288
識別號： EISSN: 1741-4288
識別號： DOI: 10.1155/2012/702857
識別號： PMID: 22701509 |
| 顯示於類別: | [生命科學系] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| index.html | | 0Kb | HTML | 21 | 檢視/開啟 |
|
在NCUIR中所有的資料項目都受到原著作權保護.
|
