Long-distance movement of Arabidopsis FLOWERING LOCUS T RNA participates in systemic floral regulation

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Title:	Long-distance movement of Arabidopsis FLOWERING LOCUS T RNA participates in systemic floral regulation
Authors:	陸重安;Lu, Kuan-Ju;Huang, Nien-Chen;Liu, Yu-Shan;Lu, Chung-An;Yu, Tien-Shin
Contributors:	生醫理工學院生命科學系
Keywords:	Arabidopsis;Arabidopsis - genetics;Arabidopsis - growth & development;Arabidopsis Proteins - genetics;Arabidopsis Proteins - metabolism;Arabidopsis Proteins - physiology;Binding;Biology;Bioscience;Calcium;Cancer;Cell;cleft grafting;Cycle;florigen;Flowers - genetics;Flowers - growth & development;FT RNA;Intercellular Signaling Peptides and Proteins - genetics;Intercellular Signaling Peptides and Proteins - metabolism;Intercellular Signaling Peptides and Proteins - physiology;Landes;loci;long-distance movement;Luminescent Proteins - genetics;Luminescent Proteins - metabolism;messenger RNA;microRNA;Organogenesis;Photoperiod;Plants, Genetically Modified;Proteins;Recombinant Fusion Proteins - genetics;Recombinant Fusion Proteins - metabolism;Red Fluorescent Protein;rice;RNA interference;RNA Transport;RNA, Messenger - genetics;RNA, Messenger - metabolism;RNA, Messenger - physiology;RNA, Plant - genetics;RNA, Plant - metabolism;RNA, Plant - physiology;scions;Seedlings - genetics;Seedlings - growth & development
Date:	2012-01-01
Issue Date:	2026-04-23 11:13:16 (UTC+8)
Publisher:	Landes Bioscience;United States: Taylor & Francis
Abstract:	摘要： The finding of mRNA acting as a systemic information molecule is one of the most exciting discoveries in recent plant biology. However, evidence demonstrating the functional significance of non-cell autonomous RNA remains limited. Recent analyses of Arabidopsis and rice revealed FLOWERING LOCUS T (FT) protein as a systemic florigenic signal. However, whether the FT RNA also participates in systemic floral regulation remains controversial. By using Arabidopsis cleft-grafting experiments, we showed that the RNA of Arabidopsis FT undergoes long-distance movement from the stock to the scion apex in both FT transformants and non-transformants. In addition, the sequences of FT RNA are sufficient to target a cell-autonomous RNA for long-distance movement. Therefore, FT RNA is a bona fide non-cell autonomous RNA. To examine the systemic action of FT RNA, we uncoupled the movement of FT RNA from protein by fusing FT with RED FLUORESCENT PROTEIN (RFP). When RFP-FT protein was retained in companion cells, the detection of RFP-FT RNA correlates with floral promotion in the scion. Further depletion of the translocated RFP-FT RNA by RNAi or artificial miRNA against FT delayed the floral promotion, indicating that the translocated FT RNA acts as a part of the systemic floral signaling. Our results indicate that both FT RNA and protein move long distance and act redundantly to integrate the photoperiodic signals. 其他題名： RNA Biol 出版者： United States: Taylor & Francis 出版日期： 2012-05-01 出處： RNA biology, 2012-05, Vol.9 (5), p.653-662 資源來源： Taylor & Francis Journals 版權： Copyright © 2012 Landes Bioscience 2012 識別號： ISSN: 1547-6286 識別號： ISSN: 1555-8584 識別號： EISSN: 1555-8584 識別號： DOI: 10.4161/rna.19965 識別號： PMID: 22614833
Appears in Collections:	[Department of Life Science] journal & Dissertation

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