Lucidone protects human skin keratinocytes against free radical-induced oxidative damage and inflammation through the up-regulation of HO-1/Nrf2 antioxidant genes and down-regulation of NF-κB signaling pathway

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Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/102587

Title:	Lucidone protects human skin keratinocytes against free radical-induced oxidative damage and inflammation through the up-regulation of HO-1/Nrf2 antioxidant genes and down-regulation of NF-κB signaling pathway
Authors:	陳師慶;Senthil Kumar, K.J.;Yang, Hsin-Ling;Tsai, Yu-Cheng;Hung, Pin-Chun;Chang, Show-Huei;Lo, Heng-Wei;Shen, Pei-Chun;Chen, Ssu-Ching;Wang, Hui-Min;Wang, Sheng-Yang;Chou, Chih-Wei;Hseu, You-Cheng
Contributors:	生醫理工學院生命科學系
Keywords:	Anti-inflammation;anti-inflammatory activity;Anti-Inflammatory Agents, Non-Steroidal - isolation & purification;Anti-Inflammatory Agents, Non-Steroidal - pharmacology;Antioxidant;antioxidant activity;antioxidants;Antioxidants - isolation & purification;Antioxidants - pharmacology;Biological and medical sciences;Cell Line;Cell Nucleus - drug effects;Cell Nucleus - metabolism;Cell Survival - drug effects;cell viability;chemokines;Cyclopentanes - isolation & purification;Cyclopentanes - pharmacology;DNA damage;DNA Damage - drug effects;Ethnopharmacology;Food toxicology;Fruit - chemistry;fruits;Gene Silencing;genes;heme oxygenase (biliverdin-producing);Heme Oxygenase-1 - chemistry;Heme Oxygenase-1 - genetics;Heme Oxygenase-1 - metabolism;Humans;inflammation;keratinocytes;Keratinocytes - drug effects;Keratinocytes - immunology;Keratinocytes - metabolism;Lindera - chemistry;Lindera erythrocarpa;lipid peroxidation;Lipid Peroxidation - drug effects;Lucidone;Medical sciences;mitogen-activated protein kinase;NF-E2-Related Factor 2 - agonists;NF-E2-Related Factor 2 - antagonists & inhibitors;NF-E2-Related Factor 2 - genetics;NF-E2-Related Factor 2 - metabolism;NF-kappa B - antagonists & inhibitors;NF-kappa B - metabolism;Nrf2;oxidative stress;Oxidative Stress - drug effects;prostaglandin synthase;prostaglandins;protective effect;Protein Transport - drug effects;reactive oxygen species;Reactive Oxygen Species - antagonists & inhibitors;Reactive Oxygen Species - metabolism;signal transduction;Signal Transduction - drug effects;Taiwan;Toxicology;transcription factor NF-kappa B;transcriptional activation
Date:	2013-09-01
Issue Date:	2026-04-23 11:13:16 (UTC+8)
Publisher:	Elsevier Ltd.;Oxford: Elsevier Ltd
Abstract:	摘要： •Lucidone protected human skin keratinocytes (HaCaT) from AAPH-induced cell death.•Lucidone inhibited AAPH-induced ROS generation, lipid peroxidation, and DNA damage.•The antioxidant potential of lucidone was directly correlated with Nrf2 activity.•Lucidone inhibited AAPH-induced inflammatory PGE2 production and COX-2 expression.•Lucidone suppressed AAPH-induced NF-κB and MAPKs signaling pathways. We investigated the protective effects of lucidone, a naturally occurring cyclopentenedione isolated from the fruits of Lindera erythrocarpa Makino, against free-radical and inflammation stimulator 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in human keratinocyte (HaCaT) cells, with the aim of revealing the possible mechanisms underlying the protective efficacy. Lucidone pretreatment (0.5–10μg/mL) markedly increased HaCaT cell viability and suppressed AAPH-induced reactive oxygen species (ROS) generation, lipid peroxidation, and DNA damage. Notably, the antioxidant potential of lucidone was directly correlated with the increased expression of an antioxidant gene, heme oxygenase 1 (HO-1), which was followed by the augmentation of the nuclear translocation and transcriptional activation of NF-E2-related factor-2 (Nrf2), with or without AAPH. Nrf2 knockdown diminished the protective effects of lucidone. We also observed that lucidone pretreatment inhibited AAPH-induced inflammatory chemokine prostaglandin E2 (PGE2) production and the expression of cyclooxygenase-2 (COX-2) in HaCaT cells. Lucidone treatment also significantly inhibited AAPH-induced nuclear factor-κB (NF-κB) activation and suppressing the degradation of inhibitor-κB (I-κB). Furthermore, lucidone significantly diminished AAPH-induced COX-2 expression through the down-regulation of the extracellular signal-regulated kinase (ERK) and p38 MAPK signaling pathways. Therefore, lucidone may possess antioxidant and anti-inflammatory properties and may be useful for the prevention of free radical-induced skin damage. 其他題名： Food Chem Toxicol 出版者： Oxford: Elsevier Ltd 出版日期： 2013-09-01 出處： Food and chemical toxicology, 2013-09, Vol.59, p.55-66 版權： 2013 Elsevier Ltd 版權： 2015 INIST-CNRS 版權： Copyright © 2013 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0278-6915 識別號： ISSN: 1873-6351 識別號： EISSN: 1873-6351 識別號： DOI: 10.1016/j.fct.2013.04.055 識別號： PMID: 23712098 識別號： CODEN: FCTOD7
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