Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae

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Title:	Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae
Authors:	陳宗祺;Wei, Tong;Chen, Tsung-Chi;Ho, Yuen Ting;Ronald, Pamela C.
Contributors:	生醫理工學院生命科學系
Keywords:	Alanine;Amino acids;Bacterial blight disease;Bacterial infections;BASIC BIOLOGICAL SCIENCES;Gene expression;Genetic aspects;Genetically modified plants;Hostages;Inoculation;Kinases;Laboratories;Localization;Mutation;Nuclear localization;Nuclei;Oryza;Pattern recognition;Plant Science;Proteins;Reactive oxygen species;Rodents;Transgenic plants;XA21-mediated immunity;Xanthomonas oryzae pv. oryzae
Date:	2016-01-01
Issue Date:	2026-04-23 11:13:29 (UTC+8)
Publisher:	PeerJ Inc.;United States: PeerJ Inc
Abstract:	摘要： Background The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods To determine if the predicted NLS is required for XA21-mediated immunity in planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results Ubi-XA21nls-GFP plants displayed slightly longer lesion lengths, higher Xoobacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi-XA21-GFP control plants. However, the Ubi-XA21nls-GFP plants express lower levels of protein than that observed in Ubi-XA21-GFP. Discussion These results demonstrate that the predicted NLS is not required for XA21-mediated immunity. 其他題名： PeerJ 出版者： United States: PeerJ Inc 出版日期： 2016-10-05 出處： PeerJ (San Francisco, CA), 2016-10, Vol.4 (10), p.e2507, Article e2507 資源來源： Directory of Open Access Journals - DOAJ (NTUSG) 版權： COPYRIGHT 2016 PeerJ. Ltd. 版權： 2016 Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2016 Wei et al. 2016 Wei et al. 識別號： ISSN: 2167-8359 識別號： EISSN: 2167-8359 識別號： DOI: 10.7717/peerj.2507 識別號： PMID: 27761320
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