Observation of &quot;wired&quot; cell communication over 10- μ m and 20- μ m poly(dimethylsiloxane) barriers in tetracycline inducible expression systems

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Title:	Observation of ";wired";cell communication over 10- μ m and 20- μ m poly(dimethylsiloxane) barriers in tetracycline inducible expression systems
Authors:	吳沛翊;Kuo, Ching-Te;Chi, Cheng-Yu;Wu, Pei-Yi;Chuang, Fang-Tzu;Lin, Yueh-Chien;Liu, Hao-Kai;Huang, Guan-Syuan;Tsai, Tzu-Ching;Wo, Andrew M.;Lee, Hsinyu;Lee, Si-Chen
Contributors:	生醫理工學院生命科學系
Keywords:	Applied physics;Barriers;Binding;Collision dynamics;Communication;Fluorescence;Kidneys;Molecular collisions;Polydimethylsiloxane;Proteins;Signal transduction;Silicone resins;Surface roughness;Thickness;Wireless communications
Date:	2016-01-14
Issue Date:	2026-04-23 11:13:33 (UTC+8)
Publisher:	American Institute of Physics;Melville: American Institute of Physics
Abstract:	摘要： Communication between cells and extracellular environments is of interest because of its critical roles in cell development and differentiation. Particularly, this signal transduction is commonly believed to rely on the contact and binding of the participating molecules/proteins, suggesting that the binding distance needed is less than a few nanometers. However, it is difficult to precisely match the rapidly binding interaction which depends on the probability of molecular collision in living systems, raising a hypothesis that another mechanism exists, could promote this signal communication, and remains unknown. Here we report that a long-range signal delivery over 10-μm and 20-μm polydimethylsiloxane (PDMS) barriers can be observed in microfluidically tetracycline (Tet) inducible expression systems. Results show that a significant increment of the long-range induced green fluorescent protein in human embryonic kidney 293T (HEK 293T) cells by the stimulation of Tet is demonstrated, and that such a signal induction is not dominated by Tet diffusion and displays a specific bindingless property. In addition, our experimental results, combined with theoretical modeling, suggest that this communication exhibits a bump-shaped characteristic depending on barrier thickness, materially structural property, surface roughness, and agonist concentration. It strongly relies on the PDMS barrier to delivery signal; therefore, we call such a mechanism as “wired” cell communication instead of wireless. These results could ignite interests in the novel and “wired” cell communication, which we call it X-signal, and in the use of such systems for the study of cellular biology and development of new drug. 出版者： Melville: American Institute of Physics 出版日期： 2016-01-14 出處： Journal of applied physics, 2016-01, Vol.119 (2) 資源來源： AIP Journals (American Institute of Physics) 版權： 2016 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License. 識別號： ISSN: 0021-8979 識別號： ISSN: 1089-7550 識別號： ISSN: 1520-8850 識別號： EISSN: 1089-7550 識別號： DOI: 10.1063/1.4939677
Appears in Collections:	[Department of Life Science] journal & Dissertation

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