Saccharomyces cerevisiae possesses a stress-inducible Glycyl-tRNA synthetase gene

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题名:	Saccharomyces cerevisiae possesses a stress-inducible Glycyl-tRNA synthetase gene
作者:	王健家;Chen, Shun-Jia;Wu, Yi-Hua;Huang, Hsiao-Yun;Wang, Chien-Chia
贡献者:	生醫理工學院生命科學系
关键词:	Amino Acid Sequence;Amino acids;Aminoacyl-tRNA synthetases;Arabidopsis thaliana;Baking yeast;Biology;E coli;Efficiency;Enzyme Induction;Enzyme Stability;Enzymes;Escherichia coli;Ethanol;Gene Knockout Techniques;Genes;Genes, Fungal;Glycine-tRNA ligase;Glycine-tRNA Ligase - biosynthesis;Glycine-tRNA Ligase - chemistry;Glycine-tRNA Ligase - genetics;Heat shock;Hydrogen;Hydrogen peroxide;Kinetics;Mitochondria;Molecular Sequence Data;Mutation;pH effects;Phaseolus vulgaris;Pichia;Plant mitochondria;Regulation;Saccharomyces cerevisiae;Saccharomyces cerevisiae - enzymology;Saccharomyces cerevisiae - genetics;Saccharomyces cerevisiae Proteins - biosynthesis;Saccharomyces cerevisiae Proteins - chemistry;Saccharomyces cerevisiae Proteins - genetics;Sequence Homology, Amino Acid;Stress, Physiological;Transfer RNA;tRNA;Yeast;Yeasts - enzymology;Yeasts - genetics
日期:	2012-03-16
上传时间:	2026-04-23 11:13:43 (UTC+8)
出版者:	Public Library of Science;United States: Public Library of Science
摘要:	摘要： Aminoacyl-tRNA synthetases are a large family of housekeeping enzymes that are pivotal in protein translation and other vital cellular processes. Saccharomyces cerevisiae possesses two distinct nuclear glycyl-tRNA synthetase (GlyRS) genes, GRS1 and GRS2. GRS1 encodes both cytoplasmic and mitochondrial activities, while GRS2 is essentially silent and dispensable under normal conditions. We herein present evidence that expression of GRS2 was drastically induced upon heat shock, ethanol or hydrogen peroxide addition, and high pH, while expression of GRS1 was somewhat repressed under those conditions. In addition, GlyRS2 (the enzyme encoded by GRS2) had a higher protein stability and a lower K(M) value for yeast tRNA(Gly) under heat shock conditions than under normal conditions. Moreover, GRS2 rescued the growth defect of a GRS1 knockout strain when highly expressed by a strong promoter at 37 °C, but not at the optimal temperature of 30 °C. These results suggest that GRS2 is actually an inducible gene that may function to rescue the activity of GRS1 under stress conditions. 其他題名： PLoS One 出版者： United States: Public Library of Science 出版日期： 2012-03-16 出處： PloS one, 2012-03, Vol.7 (3), p.e33363 資源來源： Agricultural & Environmental Science Collection 版權： COPYRIGHT 2012 Public Library of Science 版權： 2012 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： Chen et al. 2012 識別號： ISSN: 1932-6203 識別號： EISSN: 1932-6203 識別號： DOI: 10.1371/journal.pone.0033363 識別號： PMID: 22438917
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