Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice

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Title:	Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice
Authors:	許斐婷;Wang, Wei-Hsun;Chiang, I-Tsang;Liu, Yu-Chang;Hsu, Fei-Ting;Chen, Hong-Wen;Chen, Chuan-Lin;Lee, Yi-Jang;Lin, Wuu-Jyh;Hwang, Jeng-Jong
Contributors:	生醫理工學院生命科學系
Keywords:	Animals;Antineoplastic Agents - administration & dosage;Antineoplastic Agents - pharmacology;Cell Line, Tumor;Cell Survival - drug effects;Enzyme Activation - drug effects;Gene Expression;Genes, Reporter;Humans;Mice;Molecular Imaging - methods;Neoplasms - diagnosis;Neoplasms - drug therapy;Neoplasms - pathology;NF-kappa B - antagonists & inhibitors;NF-kappa B - metabolism;Niacinamide - administration & dosage;Niacinamide - analogs & derivatives;Niacinamide - pharmacology;Phenylurea Compounds - administration & dosage;Phenylurea Compounds - pharmacology;Transplantation, Heterologous;Tumor Burden - drug effects
Date:	2013-01-01
Issue Date:	2026-04-23 11:13:44 (UTC+8)
Publisher:	International Institute of Anticancer Research;Greece
Abstract:	摘要： Few studies have reported that the effect of sorafenib on advanced human hepatocellular carcinoma (HCC) is taking place via the inhibition of NF-κB signal transduction. Here we constructed a human HCC Huh7 stable clone with NF-κB-responsive element to drive dual reporter genes, herpes simplex virus thymidine kinase (tk) and firefly luciferase (luc2), and co-transfected with a third red fluorescent protein (rfp) gene, renamed as Huh7/NF-κB-tk-luc2/rfp cells, and combined with bioluminescent imaging (BLI) and red fluorescent protein imaging (RFPI) to monitor the effect of sorafenib on NF-κB activation and tumor inhibition. The results show that sorafenib could suppress the NF-κB-DNA binding activity, and the expression of downstream effector proteins. Notably, the relative photon fluxes obtained from RFPI and BLI, which represent the viable tumor cells and cells with NF-κB activation, decreased after sorafenib treatment by 50 to 65%, and 87.5 to >90%, respectively, suggesting that NF-κB activation is suppressed in viable HCC cells by sorafenib. Simultaneous molecular imaging of the temporal change of NF-κB activity and of viable cells in the same Huh7/NF-κB-tk-luc2/rfp tumors of the animal may reflect the real status of NF-κB activity and the viable tumor cells at the time of imaging. 其他題名： In Vivo 出版者： Greece 出版日期： 2013-05-01 出處： In vivo (Athens), 2013-05, Vol.27 (3), p.339 識別號： ISSN: 1791-7549 識別號： EISSN: 1791-7549 識別號： PMID: 23606689
Appears in Collections:	[Department of Life Science] journal & Dissertation

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