Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings

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Title:	Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings
Authors:	陸重安;Ho, Shin-Lon;Huang, Li-Fen;Lu, Chung-An;He, Siou-Luan;Wang, Chun-Chin;Yu, Sheng-Ping;Chen, Jychian;Yu, Su-May
Contributors:	生醫理工學院生命科學系
Keywords:	14-3-3 Proteins;14-3-3 Proteins - metabolism;Adaptation, Physiological;Adaptation, Physiological - drug effects;Adaptation, Physiological - genetics;anatomy & histology;Aquatic plants;Biochemistry;Biomedical and Life Sciences;Biosynthesis;Calcium;Carbohydrates;Carbohydrates - deficiency;cDNA libraries;cell suspension culture;complementary DNA;deficiency;Drought;Drought resistance;drought tolerance;Droughts;drug effects;Enzyme Induction;Enzyme Induction - drug effects;enzymes;enzymology;Feedback, Physiological;Feedback, Physiological - drug effects;Gene Expression Regulation, Developmental;Gene Expression Regulation, Developmental - drug effects;Gene Expression Regulation, Plant;Gene Expression Regulation, Plant - drug effects;gene overexpression;genes;genetics;germination;gibberellins;Gibberellins - biosynthesis;Gibberellins - pharmacology;growth & development;Homozygote;hormones;Kinases;Life cycles;Life Sciences;metabolism;Models, Biological;Organ Size;Organ Size - drug effects;Oryza;Oryza - drug effects;Oryza - enzymology;Oryza - genetics;Oryza - growth & development;pharmacology;physiology;Plant biology;Plant Pathology;Plant Proteins;Plant Proteins - metabolism;Plant resistance;Plant Sciences;Plants, Genetically Modified;Protein Kinases;Protein Kinases - biosynthesis;Protein Kinases - genetics;Proteins;Rice;RNA interference;seedling growth;Seedlings;Seedlings - anatomy & histology;Seedlings - drug effects;Seedlings - genetics;Seedlings - physiology;starvation;sucrose;Sugar;Up-Regulation;Up-Regulation - drug effects;water stress
Date:	2013-01-17
Issue Date:	2026-04-23 11:13:52 (UTC+8)
Publisher:	Springer Netherlands;Dordrecht: Springer Science and Business Media LLC
Abstract:	摘要： Germination followed by seedling growth constitutes two essential steps in the initiation of a new life cycle in plants, and in cereals, completion of these steps is regulated by sugar starvation and the hormone gibberellin. A calcium-dependent protein kinase 1 gene ( OsCDPK1 ) was identified by differential screening of a cDNA library derived from sucrose-starved rice suspension cells. The expression of OsCDPK1 was found to be specifically activated by sucrose starvation among several stress conditions tested as well as activated transiently during post-germination seedling growth. In gain- and loss-of-function studies performed with transgenic rice overexpressing a constitutively active or RNA interference gene knockdown construct, respectively, OsCDPK1 was found to negatively regulate the expression of enzymes essential for GA biosynthesis. In contrast, OsCDPK1 activated the expression of a 14-3-3 protein, GF14c. Overexpression of either constitutively active OsCDPK1 or GF14c enhanced drought tolerance in transgenic rice seedlings. Hence, our studies demonstrated that OsCDPK1 transduces the post-germination Ca 2+ signal derived from sugar starvation and GA, refines the endogenous GA concentration and prevents drought stress injury, all essential functions to seedling development at the beginning of the life cycle in rice. 其他題名： Plant Mol Biol 出版者： Dordrecht: Springer Science and Business Media LLC 出版日期： 2013-03-01 出處： Plant Molecular Biology, 2013-03, Vol.81 (4), p.347-361 版權： Springer Science+Business Media Dordrecht 2013 識別號： ISSN: 0167-4412 識別號： ISSN: 1573-5028 識別號： EISSN: 1573-5028 識別號： DOI: 10.1007/s11103-012-0006-z 識別號： PMID: 23329372
Appears in Collections:	[Department of Life Science] journal & Dissertation

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