The anti-cancer activity of Antrodia camphorata against human ovarian carcinoma (SKOV-3) cells via modulation of HER-2/neu signaling pathway

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Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/102634

Title:	The anti-cancer activity of Antrodia camphorata against human ovarian carcinoma (SKOV-3) cells via modulation of HER-2/neu signaling pathway
Authors:	陳師慶;Yang, Hsin-Ling;Lin, Kai-Yuan;Juan, Ying-Chen;Kumar, K.J. Senthil;Way, Tzong-Der;Shen, Pei-Chun;Chen, Ssu-Ching;Hseu, You-Cheng
Contributors:	生醫理工學院生命科學系
Keywords:	animal ovaries;anticarcinogenic activity;Antineoplastic Agents;Antineoplastic Agents - pharmacology;Antrodia;Antrodia camphorate;Apoptosis;Apoptosis - drug effects;carcinoma;cell death;cell growth;Cell Line, Tumor;Cell Proliferation;Cell Proliferation - drug effects;cell viability;Complex Mixtures;Complex Mixtures - pharmacology;cytochrome c;DNA fragmentation;drug effects;Female;flow cytometry;fluorescence microscopy;fluorescent antibody technique;fungi;G2/M arrest;HER-2/neu;Humans;image analysis;metabolism;Ovarian cancer;Ovarian Neoplasms;pharmacology;phosphorylation;reactive oxygen species;Reactive Oxygen Species - metabolism;Receptor, ErbB-2;Receptor, ErbB-2 - metabolism;ROS;Signal Transduction;Signal Transduction - drug effects;submerged fermentation;Taiwan;Taiwanofungus camphoratus;tyrosine;Western blotting
Date:	2013-06-21
Issue Date:	2026-04-23 11:13:59 (UTC+8)
Publisher:	Ireland: Elsevier Ireland Ltd
Abstract:	摘要： Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicinal fungus. However, the anticancer activity of AC against human HER-2/neu-overexpressing ovarian cancers is poorly understood. The aim of this study is to investigate whether a submerged fermentation culture of AC can inhibit human ovarian carcinoma cell (SKOV-3) proliferation by suppressing the HER-2/neu signaling pathway. Cell viability, colony formation, DCFH-DA fluorescence microscopy, western blotting, HER-2/neu immunofluorescence imaging, flow cytometry, and TUNEL assays were carried out to determine the anti-cancer effects of AC. MTT and colony formation assays showed that AC induced a dose-dependent reduction in SKOV-3 cell growth. Immunoblot analysis demonstrated that HER-2/neu activity and tyrosine phosphorylation were significantly inhibited by AC. Furthermore, AC treatment significantly inhibited the activation of PI3K/Akt and their downstream effector β-catenin. We also observed that AC caused G2/M arrest mediated by down-regulation of cyclin D1, cyclin A, cyclin B1, and Cdk1 and increased p27 expression. Notably, AC induced apoptosis, which was associated with DNA fragmentation, cytochrome c release, caspase-9/-3 activation, PARP degradation, and Bcl-2/Bax dysregulation. An increase in intracellular reactive oxygen species (ROS) was observed in AC-treated cells, whereas the antioxidant N-acetylcysteine (NAC) prevented AC-induced cell death, HER-2/neu depletion, PI3K/Akt inactivation, and Bcl-2/Bax dysregulation, indicating that AC-induced cell death was mediated by ROS generation. These results suggest that AC may exert anti-tumor activity against human ovarian carcinoma by suppressing HER-2/neu signaling pathways. The submerged fermentation culture of Antrodia camphorata (AC) could inhibit the human ovarian carcinoma (SKOV-3) proliferation by suppressing the HER-2/neu signaling pathway [Display omitted] . 其他題名： J Ethnopharmacol 出版者： Ireland: Elsevier Ireland Ltd 出版日期： 2013-06-21 出處： Journal of Ethnopharmacology, 2013-06, Vol.148 (1), p.254-265 版權： 2013 Elsevier Ireland Ltd 版權： Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. 識別號： ISSN: 0378-8741 識別號： ISSN: 1872-7573 識別號： EISSN: 1872-7573 識別號： DOI: 10.1016/j.jep.2013.04.023 識別號： PMID: 23619020
Appears in Collections:	[Department of Life Science] journal & Dissertation

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