Two highly similar DEAD box proteins, OsRH2 and OsRH34, homologous to eukaryotic initiation factor 4AIII, play roles of the exon junction complex in regulating growth and development in rice

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题名:	Two highly similar DEAD box proteins, OsRH2 and OsRH34, homologous to eukaryotic initiation factor 4AIII, play roles of the exon junction complex in regulating growth and development in rice
作者:	陸重安;Huang, Chun-Kai;Sie, Yi-Syuan;Chen, Yu-Fu;Huang, Tian-Sheng;Lu, Chung-An
贡献者:	生醫理工學院生命科學系
关键词:	Agriculture;Amino Acid Sequence;amino acid sequences;Amino acids;Analysis;Biomedical and Life Sciences;Cell Nucleus - metabolism;cytoplasm;Cytoplasm - metabolism;DEAD-box RNA helicases;DEAD-box RNA Helicases - genetics;DEAD-box RNA Helicases - metabolism;Development and cell biology;dwarfing;embryogenesis;Embryonic growth stage;Eukaryotic Initiation Factor-4A - classification;Eukaryotic Initiation Factor-4A - genetics;exons;Exons - genetics;fluorescence;Gene expression;Gene Expression Profiling - methods;Gene Expression Regulation, Developmental;Gene Expression Regulation, Enzymologic;Gene Expression Regulation, Plant;Influence;Isoenzymes - genetics;Isoenzymes - metabolism;Life Sciences;Luminescent Proteins - genetics;Luminescent Proteins - metabolism;lymph nodes;Messenger RNA;metastasis;Microscopy, Fluorescence;mutants;Oryza - genetics;Oryza - growth & development;Oryza - metabolism;Oryza sativa;phenotype;Phylogeny;Plant Proteins - classification;Plant Proteins - genetics;Plant Proteins - metabolism;Plant Sciences;Plants, Genetically Modified;Pollen;Pollen - genetics;Pollen - growth & development;Pollen - metabolism;Protein Binding;Research Article;Reverse Transcriptase Polymerase Chain Reaction;rice;RNA Interference;seed set;Seedlings;Seeds - genetics;Seeds - growth & development;Seeds - metabolism;sequence analysis;Sequence Homology, Amino Acid;tissues;transgenic plants;Tree Biology
日期:	2016-01-01
上传时间:	2026-04-23 11:14:16 (UTC+8)
出版者:	BioMed Central Ltd.;London: BioMed Central
摘要:	摘要： Background The exon junction complex (EJC), which contains four core components, eukaryotic initiation factor 4AIII (eIF4AIII), MAGO/NASHI (MAGO), Y14/Tsunagi/RNA-binding protein 8A, and Barentsz/Metastatic lymph node 51, is formed in both nucleus and cytoplasm, and plays important roles in gene expression. Genes encoding core EJC components have been found in plants, including rice. Currently, the functional characterizations of MAGO and Y14 homologs have been demonstrated in rice. However, it is still unknown whether eIF4AIII is essential for the functional EJC in rice. Results This study investigated two DEAD box RNA helicases, OsRH2 and OsRH34, which are homologous to eIF4AIII, in rice. Amino acid sequence analysis indicated that OsRH2 and OsRH34 had 99 % identity and 100 % similarity, and their gene expression patterns were similar in various rice tissues, but the level of OsRH2 mRNA was about 58-fold higher than that of OsRH34 mRNA in seedlings. From bimolecular fluorescence complementation results, OsRH2 and OsRH34 interacted physically with OsMAGO1 and OsY14b, respectively, which indicated that both of OsRH2 and OsRH34 were core components of the EJC in rice. To study the biological roles of OsRH2 and OsRH34 in rice, transgenic rice plants were generated by RNA interference. The phenotypes of three independent OsRH2 and OsRH34 double-knockdown transgenic lines included dwarfism, a short internode distance, reproductive delay, defective embryonic development, and a low seed setting rate. These phenotypes resembled those of mutants with gibberellin-related developmental defects. In addition, the OsRH2 and OsRH34 double-knockdown transgenic lines exhibited the accumulation of unspliced rice UNDEVELOPED TAPETUM 1 mRNA. Conclusions Rice contains two eIF4AIII paralogous genes, OsRH2 and OsRH34 . The abundance of OsRH2 mRNA was about 58-fold higher than that of OsRH34 mRNA in seedlings, suggesting that the OsRH2 is major eIF4AIII in rice. Both OsRH2 and OsRH34 are core components of the EJC, and participate in regulating of plant height, pollen, and seed development in rice. 其他題名： BMC Plant Biol 出版者： London: BioMed Central 出版日期： 2016-04-12 出處： BMC plant biology, 2016-04, Vol.16 (1), p.84-84, Article 84 資源來源： Agricultural & Environmental Science Collection 版權： Huang et al. 2016 版權： COPYRIGHT 2016 BioMed Central Ltd. 版權： Copyright BioMed Central 2016 識別號： ISSN: 1471-2229 識別號： EISSN: 1471-2229 識別號： DOI: 10.1186/s12870-016-0769-5 識別號： PMID: 27071313
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