5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary

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Title:	5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary
Authors:	王孫崇;Khare, Tarang;Pai, Shraddha;Koncevicius, Karolis;Pal, Mrinal;Kriukiene, Edita;Liutkeviciute, Zita;Irimia, Manuel;Jia, Peixin;Ptak, Carolyn;Xia, Menghang;Tice, Raymond;Tochigi, Mamoru;Moréra, Solange;Nazarians, Anaies;Belsham, Denise;Wong, Albert H C;Blencowe, Benjamin J;Wang, Sun Chong;Kapranov, Philipp;Kustra, Rafal;Labrie, Viviane;Klimasauskas, Saulius;Petronis, Arturas
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	5-Methylcytosine;5-Methylcytosine - metabolism;631/337/1645/1946;631/337/572;631/378/340;Alternative Splicing;Animals;Biochemistry;Biological Microscopy;Biomedical and Life Sciences;Brain;Brain - physiology;Cell Line;Cytosine;Cytosine - analogs & derivatives;Cytosine - metabolism;Deoxyribonucleic acid;DNA;DNA probes;DNA sequencing;Genetic aspects;Genetic engineering;Genomics;Glucosyltransferases;Glucosyltransferases - metabolism;Humans;Introns;Life Sciences;Male;Membrane Biology;Methods;Mice;Mice, Inbred C57BL;Microarray Analysis;Molecular biology;Neural transmission;Nucleotide sequencing;Organ Specificity;Protein Structure;Reproducibility of Results;resource;RNA Splicing;Rodents;Synapses;Synapses - genetics;Synapses - metabolism
Date:	2012-10-01
Issue Date:	2026-04-23 11:14:33 (UTC+8)
Publisher:	Nature Publishing Group;New York: Springer Science and Business Media LLC
Abstract:	摘要： The 5-methylcytosine (5-mC) derivative 5-hydroxymethylcytosine (5-hmC) is abundant in the brain for unknown reasons. Here we characterize the genomic distribution of 5-hmC and 5-mC in human and mouse tissues. We assayed 5-hmC by using glucosylation coupled with restriction-enzyme digestion and microarray analysis. We detected 5-hmC enrichment in genes with synapse-related functions in both human and mouse brain. We also identified substantial tissue-specific differential distributions of these DNA modifications at the exon-intron boundary in human and mouse. This boundary change was mainly due to 5-hmC in the brain but due to 5-mC in non-neural contexts. This pattern was replicated in multiple independent data sets and with single-molecule sequencing. Moreover, in human frontal cortex, constitutive exons contained higher levels of 5-hmC relative to alternatively spliced exons. Our study suggests a new role for 5-hmC in RNA splicing and synaptic function in the brain. 其他題名： Nat Struct Mol Biol 出版者： New York: Springer Science and Business Media LLC 出版日期： 2012-10-01 出處： Nature Structural & Molecular Biology, 2012-10, Vol.19 (10), p.1037-1043 資源來源： OmniFile Full Text Select(H.W. Wilson)-EBSCOhost全文資料庫版權： Springer Nature America, Inc. 2012 版權： COPYRIGHT 2012 Nature Publishing Group 版權： Copyright Nature Publishing Group Oct 2012 識別號： ISSN: 1545-9993 識別號： ISSN: 1545-9985 識別號： EISSN: 1545-9985 識別號： DOI: 10.1038/nsmb.2372 識別號： PMID: 22961382
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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