A precision microbiome approach using sucrose for selective augmentation of Staphylococcus epidermidis fermentation against Propionibacterium acnes

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Title:	A precision microbiome approach using sucrose for selective augmentation of Staphylococcus epidermidis fermentation against Propionibacterium acnes
Authors:	黃俊銘;Wang, Yanhan;Kao, Ming-Shan;Yu, Jinghua;Huang, Stephen;Marito, Shinta;Gallo, Richard;Huang, Chun-Ming
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Acne;Acne Vulgaris - microbiology;Acne Vulgaris - pathology;Acne Vulgaris - therapy;Animals;Antibiosis;Bacterial Load - drug effects;Biomarkers - metabolism;Chemokine CXCL2 - genetics;Chemokine CXCL2 - metabolism;Coculture Techniques;Dysbiosis - microbiology;Dysbiosis - pathology;Dysbiosis - therapy;Ear - microbiology;Ear - pathology;Female;Fermentation - drug effects;Gene Expression;Glycerol - metabolism;Glycerol - pharmacology;Humans;Mice;Mice, Inbred ICR;Microbiota;Probiotics - metabolism;Probiotics - pharmacology;Propionibacterium acnes;Propionibacterium acnes - drug effects;Propionibacterium acnes - growth & development;Propionibacterium acnes - pathogenicity;Skin;Skin - drug effects;Skin - microbiology;Skin - pathology;Staphylococcus;Staphylococcus epidermidis;Staphylococcus epidermidis - drug effects;Staphylococcus epidermidis - growth & development;Staphylococcus epidermidis - metabolism;Staphylococcus infections;Sucrose;Sucrose - metabolism;Sucrose - pharmacology
Date:	2016-11-01
Issue Date:	2026-04-23 11:14:53 (UTC+8)
Publisher:	MDPI Multidisciplinary Digital Publishing Institute;Switzerland: MDPI AG
Abstract:	摘要： Acne dysbiosis happens when there is a microbial imbalance of the over-growth of Propionibacterium acnes (P. acnes) in the acne microbiome. In our previous study, we demonstrated that Staphylococcus epidermidis (S. epidermidis, a probiotic skin bacterium) can exploit glycerol fermentation to produce short-chain fatty acids (SCFAs) which have antimicrobial activities to suppress the growth of P. acnes. Unlike glycerol, sucrose is chosen here as a selective fermentation initiator (SFI) that can specifically intensify the fermentation activity of S. epidermidis, but not P. acnes. A co-culture of P. acnes and fermenting S. epidermidis in the presence of sucrose significantly led to a reduction in the growth of P. acnes. The reduction was abolished when P. acnes was co-cultured with non-fermenting S. epidermidis. Results from nuclear magnetic resonance (NMR) analysis revealed four SCFAs (acetic acid, butyric acid, lactic acid, and succinic acid) were detectable in the media of S. epidermidis sucrose fermentation. To validate the interference of S. epidermidis sucrose fermentation with P. acnes, mouse ears were injected with both P. acnes and S. epidermidis plus sucrose or phosphate buffered saline (PBS). The level of macrophage-inflammatory protein-2 (MIP-2) and the number of P. acnes in ears injected with two bacteria plus sucrose were considerably lower than those in ears injected with two bacteria plus PBS. Our results demonstrate a precision microbiome approach by using sucrose as a SFI for S. epidermidis, holding future potential as a novel modality to equilibrate dysbiotic acne. 其他題名： Int J Mol Sci 出版者： Switzerland: MDPI AG 出版日期： 2016-11-09 出處： International Journal of Molecular Sciences, 2016-11, Vol.17 (11), p.1870--1870 資源來源： ProQuest Open Access Content Collection 版權： Copyright MDPI AG 2016 版權： 2016 by the authors; licensee MDPI, Basel, Switzerland. 2016 識別號： ISSN: 1422-0067 識別號： ISSN: 1661-6596 識別號： EISSN: 1422-0067 識別號： DOI: 10.3390/ijms17111870 識別號： PMID: 27834859
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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